PhD Scientific Days 2020

Theoretical and Translational III. Posters

Protective Role of Sigma-1 Receptor in Corneal Fibrosis

Előadó neve

Szabó, Adrienn, MSc

Előadó munkahelye

1st Dept. of Pediatrics, Semmelweis University

Előadó telefonszáma

+36(20)461-5158

Előadó e-mail címe

szabo.adrienne93@gmail.com

Az előadás címe

Protective Role of Sigma-1 Receptor in Corneal Fibrosis

Szerző(k) neve és munkahelye

Adrienn Szabo (1,2), Lilla Lenart (1,2), Judit Hodrea (1,2), Balazs Besztercei (3), Illes Kovacs (4), Attila J. Szabo (2,5), Andrea Fekete (1,2)
1 MTA-SE Lendület Diabetes Research Group, Budapest
2 SE 1st Department of Paediatrics, Budapest
3 Institute of Clinical Experimental Research, Semmelweis University, Budapest
4 SE Department of Ophthalmology, Budapest
5 MTA-SE Pediatrics and Nephrology Research Group, Budapest

Szekció

Theoretical and Translational III. Posters

Language of the presentation

Hungarian

Section, first choice

Theoretical and Translational Medicine

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction
Corneal scarring is a pivotal cause of visual impairment, which accounts for over 4% of blindness worldwide with 2 million cases each year. Scarring of the corneal tissue, also known as fibrosis, commonly occurs after infection, postsurgical complications, and response to injury or metabolic disease. Corneal fibrosis is characterized by the presence of myofibroblasts and deposition of extracellular matrix components. Currently, treatment options for cornea scarring are limited and the only effective treatment is corneal transplantation.
Our research group proved that activation of Sigma-1 receptor (S1R) by fluvoxamine (Flu) has antifibrotic effect in the kidney and lung, therefore we hypothesize that Flu could have a protective role in the prevention of fibrosis in the cornea as well.
Aims
To optimize the methods and culturing techniques of primary fibroblast cells from the cornea. To detect the presence of S1R in primary corneal fibroblasts and to measure the effect of Flu on platelet-derived growth factor (PDGF-BB) or transforming growth factor-beta1 (TGF-β1) -induced proliferation and migration of corneal fibroblast cells.
Methods
Cells were treated with PDGF-BB or TGF-β1 to induce fibrotic process. S1R (localization), F-actin and α-SMA (for morphological changes/cytoskeleton rearrangements) were detected by immunofluorescence staining. Cell proliferation was evaluated by thiazolyl blue tetrazolium bromide (MTT) method. Cell migration were followed by wound healing assay.
Results
Primary corneal fibroblast cell culturing method was optimized in three species (human, rat and mouse). S1R is localized in the cytosol of corneal fibroblast cells. PDGF-BB and TGF-β1 increased the proliferation and migration of corneal fibroblast cells and promoted the transformation of fibroblasts to myofibroblasts. These effects were inhibited by Flu.
Conclusion
Flu reduces cytoskeletal rearrangement, myofibroblast transformation and migration, indicating its antifibrotic properties. Our preliminary results propose that Flu could be a potential candidate for development of a novel drug reducing fibrosis in the cornea. However, further molecular experiments (such as investigating inflammatory and apoptotic pathways) and in vivo animal models are needed to reinforce these results.

Grants: NNOTKA-114607, VKE-2017-00006, FK124491

Additional Information

Supervisor: Lilla Lenart
E-mail address: lenart.lillaa@gmail.com

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4758

Start

11:10

End

11:13

Authors (legacy)

Adrienn Szabo (1,2), Lilla Lenart (1,2), Judit Hodrea (1,2), Balazs Besztercei (3), Illes Kovacs (4), Attila J. Szabo (2,5), Andrea Fekete (1,2)
1 MTA-SE Lendület Diabetes Research Group, Budapest
2 SE 1st Department of Paediatrics, Budapest
3 Institute of Clinical Experimental Research, Semmelweis University, Budapest
4 SE Department of Ophthalmology, Budapest
5 MTA-SE Pediatrics and Nephrology Research Group, Budapest