PhD Scientific Days 2020

Neurosciences I. Posters

Examination of the Reorganisation of Parvalbumin Positive Perisomatic Innervation in Different Cortical Areas of Dysgenetic Samples of Human Epileptic Patients

Előadó neve

Paraczky, Cecília, MSc

Előadó munkahelye

Institute of Experimental Medicine

Előadó telefonszáma

304387838

Előadó e-mail címe

paraczky.cili@gmail.com

Az előadás címe

Examination of the Reorganisation of Parvalbumin Positive Perisomatic Innervation in Different Cortical Areas of Dysgenetic Samples of Human Epileptic Patients

Szerző(k) neve és munkahelye

Cecília Paraczky1, Péter Szocsics1, Loránd Erőss2, Dániel Fabó2, László Havas3, Zsófia Maglóczky1,4
1Human Brain Research Laboratory, Institute of Experimental Medicine, Budapest
2National Institute of Clinical Neuroscience, Budapest
3St. Borbála Hospital, Tatabánya
4Laboratory of Cerebral Cortex, Institute of Experimental Medicine, HAS, Budapest

Szekció

Neurosciences I. Posters

Language of the presentation

Hungarian

Section, first choice

Neurosciences

Section, second choice

Neurosciences

Összefoglaló szövege

Introduction:
Drug resistant epilepsy is often associated with focal cortical dysplasia (FCD).FCD is a neurodevelopmental disorder with diverse pathological changes, such as the appearance of abnormal cell types and impaired cortical lamination. Type II FCDs are characterized by the presence of morphologically abnormal cells, including cytomegalic dysmorphic neurons or balloon cells and abnormal glial cells besides the different degrees of disruption of the cortical lamination. In TLE enhanced perisomatic inhibition was found which may increase synchronous firing of principal cells and seizure probability.Therefore, we wanted to investigate, whether changes of perisomatic inhibitory inputs can be found in epilepsy with FCD background, suggesting the possibility of similar patomechanism in different types of epilepsies.
Methods:Surgical samples from FCD patients (FCDIIb, 6 cases: 4 frontal, 1 parietal, and 1 occipital cortex) were compared to control samples of the same cortical regions with short post mortem interval (2-5 h, perfusion fixation) (6 control subjects: 4 samples from frontal, 1 from parietal, and 1 from occipital area).The perisomatic inhibitory terminals contacting principal cells were examined with PV-immunostaining and quantified by PV-NeuN double immunostaining in confocal fluorescent microscope.
Results:The pathological pattern of epileptic patients with FCD was heterogeneous from mostly control-like tissue to disorganized cortical layers and abnormal cells. The current study was performed on samples with control-like layers.Despite the individual variations, comparison of pooled data of PV-immunopositive synaptic coverage of principal cells from control and epileptic subjects showed that the number of PV-immunostained terminals/unit of perimeter is significantly larger in FCD cases.
Conclusion:It is unclear that the amount of PV or PV-stained inhibitory elements are changing due to the adaptive mechanism balancing the excessive electrical discharges, or is a prior pathological alteration that is further increasing by the effects of seizures. However, the change of the perisomatic inhibitory system in both cases could further increase the probability of seizure activity. Therefore, the alteration of perisomatic inhibition of principal cells may be a general mechanism of abnormal network activity, because it was observed in TLE as well.

Additional Information

Maglóczky Zsófia
magloczky.zsofia@koki.mta.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4773

Start

12:59

End

13:02

Authors (legacy)

Cecília Paraczky1, Péter Szocsics1, Loránd Erőss2, Dániel Fabó2, László Havas3, Zsófia Maglóczky1,4
1Human Brain Research Laboratory, Institute of Experimental Medicine, Budapest
2National Institute of Clinical Neuroscience, Budapest
3St. Borbála Hospital, Tatabánya
4Laboratory of Cerebral Cortex, Institute of Experimental Medicine, HAS, Budapest