PhD Scientific Days 2020

Pharmaceutical Sciences II. Lectures

Application of Amino Acid Based Polymers for Drug Delivery

Előadó neve

Tóth, Krisztina, MSc

Előadó munkahelye

Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest; Department of Oral Biology, Semmelweis University, Budapest

Előadó telefonszáma

+36707766458

Előadó e-mail címe

toth.krisztina.105@gmail.com

Az előadás címe

Application of Amino Acid Based Polymers for Drug Delivery

Szerző(k) neve és munkahelye

Krisztina Tóth1,2, Dávid Juriga1, Angéla Jedlovszky-Hajdú1, Miklós Zrínyi1, Gábor Varga2, Krisztina S. Nagy1

1 Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
2 Department of Oral Biology, Semmelweis University, Budapest

Szekció

Pharmaceutical Sciences II. Lectures

Language of the presentation

Hungarian

Section, first choice

Pharmaceutical Sciences

Section, second choice

Neurosciences

Összefoglaló szövege

Biopolymers such as poly(aspartamide) with high degree of polymerization are suitable materials for preparing drug delivery systems. Poly(succinimide), which is the anhydrous form of poly(aspartid acid), is chemically similar to the natural proteins, therefore it possess excellent degradation property at physiological condition. Moreover, it could provide reduced side effects and protect the drug from early deactivation. Applying electrospinning technique, fibrous polymer meshes can be produced with a diameter in the nano- or micrometer range. Due to the large specific surface, they can increase the dissolution and absorption of the conjugated drug. As a consequence of these favorable properties, better pharmaceutical theraphy can be achieved.
The aim of this study was to fabricate biocompatible and biodegradable, nanofibrous polymer-drug conjugates using dopamine-conjugated poly(aspartamid) and to investigate the incidental citotoxic effect of these systems.
In order to prepare polymer-drug conjugates, poly(succinimide) was conjugated with dopamine and formulated by electrospinning. The electrospun meshes were characterized both chemically (FT-IR) and physically (scanning electon microscopy, atomic force and two-photon microscopy). The kinetics of dopamine release and the solubility were monitored by UV-VIS spectroscopy. The biocompatibility was assessed by culturing of human dental stem cells and the SH-SY5Y human neuroblastoma cell line in the presence of these polymer-dopamine conjugates. The cell viability was determined using the WST-1 proliferation reagent and cell morphology was observed under phase-contrast, two-photon as well as confocal microscopy. The presence of dopamine receptors on both cell types were investigated by immunocytochemical analysis.
Nanofibrous meshes were successfully prepared from dopamine-conjugated poly(aspartamide) and prolonged drug release was revealed. According to the cell viability results, these conjugates are biocompatible. The cells tolerate the dopamine-polymer conjugates until a higher concentration than free dopamine.
Applying these biocompatible poly(aspartamide) based drug delivery systems, the side-effects can be reduced, which is a promising feature regarding the future therapy.
This research was supported by UNKP-17-3-III-SE-17, EFOP-3.6.2-06-2017-00006, OTKA-NKTH CK-80928, OTKA-K115259, NKFI FK 124147.

Additional Information

Supervisor: Gábor Varga
Supervisor's e-mail adress: varga.gabor@dent.semmelweis-univ.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4183

Start

12:25

End

12:40

Authors (legacy)

Krisztina Tóth1,2, Dávid Juriga1, Angéla Jedlovszky-Hajdú1, Miklós Zrínyi1, Gábor Varga2, Krisztina S. Nagy1

1 Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
2 Department of Oral Biology, Semmelweis University, Budapest