PhD Scientific Days 2020

Clinical Medicine III. Posters

New Perspectives on Non-culture Based Diagnostics of Community-acquired Sepsis: a pilot study from Bedside to Benchmark

Előadó neve

Dr. Szabó, Balint Gergely

Előadó munkahelye

South Pest Central Hospital, National Institute of Hematology and Infectious Diseases; Institute of Medical Microbiology

Előadó telefonszáma

06306445976

Előadó e-mail címe

szabo.balint.gergely@gmail.com

Az előadás címe

New Perspectives on Non-culture Based Diagnostics of Community-acquired Sepsis: a pilot study from Bedside to Benchmark

Szerző(k) neve és munkahelye

Balint Gergely SZABO1,2, Nora MAKRA2, Eszter OSTORHAZI2
1 South Pest Central Hospital, National Institute of Hematology and Infectious Diseases, Budapest
2 Institute of Medical Microbiology, Semmelweis University, Budapest

Szekció

Clinical Medicine III. Posters

Language of the presentation

Hungarian

Section, first choice

Clinical Medicine

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction
Community-acquired sepsis (CAS) is a potentially fatal systemic reaction to infection starting ≤72 hours after hospital admittance. Clinical and microbiological data concerning CAS, especially among Hungarian patients are sparse. Furthermore, the causing agents of CAS are not identifiable by routine microbiological methods in some patients.
Aims
Our aim was 1) to identify adult CAS patients from a large cohort who did not have any causative agents indentified during hospital stay by routine microbiological methods (culturing, PCR, serology), 2) measure the rate of this phenomenon and 3) assess the usefulness of 16S rRNA metagenomic sequencing analysis in the identification of CAS etiologies.
Methods
This pilot study had 2 phases. In the first phase, a retrospective cohort study was done among a cohort of adult CAS patients admitted to a national referral center during 2016. In the second phase, whole blood samples were taken from randomly selected CAS patients for 16S rRNA metagenomic sequencing analysis. Patients with possible healthcare associations were excluded, sepsis definitions and severity were given according to ACCP/SCCM SIRS-criteria.
Results
In the first phase, 206 patients were included (mean age 58.4±20.4 years, 56.7% female). Estimated incidence of CAS was 285 / 10.000 admittances per year. 66/206 (32.0%) cases were severe sepsis, and 59/206 (28.6%) were septic shock. The identified causative organisms were mainly E. coli (56/216, 25.9%), S. aureus (14/216, 6.5%) and S. pneumoniae (15/216, 6.9%), and in quarter of all cases, no organism could be identified (54/216, 25.0%). Bacteraemia was proven in half of cases (104/206, 50.5%). In the second phase, blood samples of 15 patients are being analysed at the moment. 5 patients did not have any causative organisms identified.
Conclusion
A relevant number of CAS patients do not have a causative agent identified by routine microbiological methods. we hope that 16S rRNA metagenomic sequencing analysis might have a role in the identification of these infections.

Additional Information

Supervisor: droeszter@gmail.com
The first author is a granted PhD student of the New Excellency Scolarship.

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4182

Start

12:37

End

12:40

Authors (legacy)

Balint Gergely SZABO1,2, Nora MAKRA2, Eszter OSTORHAZI2
1 South Pest Central Hospital, National Institute of Hematology and Infectious Diseases, Budapest
2 Institute of Medical Microbiology, Semmelweis University, Budapest