PhD Scientific Days 2020

Neurosciences II. Lectures

Early-Life Social Isolation is Linked to Abnormal Social Behaviour in Adulthood and Disrupted Network Organization in the Prefrontal Cortex

Előadó neve

Miskolczi, Christina, MSc

Előadó munkahelye

Institute of Experimental Medicine

Előadó telefonszáma

06303800907

Előadó e-mail címe

miskolczi.christina@koki.mta.hu

Az előadás címe

Early-Life Social Isolation is Linked to Abnormal Social Behaviour in Adulthood and Disrupted Network Organization in the Prefrontal Cortex

Szerző(k) neve és munkahelye

Christina Miskolczi1, Laszlo Biro1, Biborka Bruzsik1, Huba Szebik1, David Lorincz1, Zoltan Kristof Varga1, Laszlo Szente1, Orsolya Horvath1, Jozsef Halasz1, Mate Toth1, Eva Mikics1
1 Institute of Experimental Medicine, Budapest

Szekció

Neurosciences II. Lectures

Language of the presentation

English

Section, first choice

Neurosciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Brain regions modulating social behaviour undergo dynamic changes in early life, rendering them vulnerable to social adversities experienced during this period. Aims: We aimed to characterize social behavioural changes induced by post-weaning social isolation (a model of childhood neglect) in mice and investigate underlying network disturbances in the prefrontal cortex (PFC), a key modulator of social behaviour. We focused on parvalbumin (PV) interneurons, a neuronal population closely tied to critical period closure. Methods: Mice were weaned at postnatal day 21 and were housed either socially (4 mice/cage) or were isolated (alone) until adulthood. In adulthood, we used the resident-intruder (RI) test to investigate social behaviour and aggression. Mice were perfused either under resting conditions (no RI) or 90 minutes following social encounter (RI). Using immunohistochemistry and confocal imaging, we investigated (1) general c-Fos activation and co-activation patterns of PFC subregions, (2) activation of PV-containing (PV+) and perineuronal net-surrounded (PNN+) neurons in each subregion under resting conditions or following social encounter (RI) and (3) changes in general and excitatory inputs to the soma of parvalbumin interneurons in social and isolated animals via Bassoon and vGlut1 staining. Results: Isolated mice display social disturbances in adulthood, manifested as increased defensive and abnormal aggressive behavior disregarding species-specific rules. Correlation matrices reveal that social encounter exerts differential c-Fos activation patterns in the PFC of isolated animals. In dorsal PFC regions RI increased the activity of PV+PNN+ neurons in both social and isolated animals. Conversely, in the infralimbic cortex social encounter decreased the activity of PV+PNN+ neurons in socially-reared mice, an effect absent in isolated animals. Isolation also lead to a decrease in Bassoon+ and vGlut1+ puncta surrounding parvalbumin interneurons. In conclusion, social isolation leads to social behavioural abnormalities and impaired activity of PV+PNN+ neurons following social encounter. Changes in inputs to parvalbumin interneurons could contribute to their abnormal activity. Our results aid us in understanding how early-life adversities disrupt developmental processes, leading to social abnormalities in adulthood.

Additional Information

Supervisor: Eva Mikics
E-mail address: mikics.eva@koki.mta.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4793

Start

17:15

End

17:30

Authors (legacy)

Christina Miskolczi1, Laszlo Biro1, Biborka Bruzsik1, Huba Szebik1, David Lorincz1, Zoltan Kristof Varga1, Laszlo Szente1, Orsolya Horvath1, Jozsef Halasz1, Mate Toth1, Eva Mikics1
1 Institute of Experimental Medicine, Budapest