PhD Scientific Days 2020

Neurosciences I. Posters

P2Y12 Receptors are Essential for Physiological Tissue Distribution, 3D Morphology and Motility of Microglia

Előadó neve

Dr. Pósfai, Balázs

Előadó munkahelye

Lendület Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest, Hungary

Előadó e-mail címe

posfai.balazs@koki.mta.hu

Az előadás címe

P2Y12 Receptors are Essential for Physiological Tissue Distribution, 3D Morphology and Motility of Microglia

Szerző(k) neve és munkahelye

Balázs Pósfai1,2, Csaba Cserép1, Rebeka Fekete1, Krisztina Tóth1,2, Anett Dóra Schwarcz1, Katinka Ujvári1, Ádám Dénes1
1 Lendület Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest, Hungary
2 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary

Szekció

Neurosciences I. Posters

Language of the presentation

English

Section, first choice

Neurosciences

Section, second choice

Neurosciences

Összefoglaló szövege

There is growing evidence indicating the importance of microglia in maintaining proper neuronal activity and function. During their surveillance activity, motile microglial processes constantly monitor their environment, while purinergic signalling via the P2Y12 receptor (P2Y12R) is required for microglial process recruitment to sites of injury and for the elimination of terminally injured cells by microglia in several pathological states. These receptors are also enriched in somatic microglial junctions. Although P2Y12R is microglia-specific in the brain, the role of P2Y12R signalling in microglial responses and surveillance activity under physiological conditions remained largely unexplored. Thus, the aim of our study was to examine the role of P2Y12R signalling in microglial morphology and function under physiological conditions in mice by examining the effect of genetic deletion and acute pharmacological inhibition of P2Y12R.
The lack of microglial P2Y12R resulted in significant disturbances of microglial localization in the neocortex of mice. P2Y12R-/- animals had elevated density of microglial cells, while the ratio of satellite microglia was also significantly increased. The absence of P2Y12R function altered microglial morphology, also showing differences between acute and chronic blockade. In addition, selective blockade of P2Y12R drastically decreased the motility of surveilling microglial processes in vivo.
Our data indicate that P2Y12R function is essential for proper microglial actions even under physiological conditions. Disturbance of P2Y12R signalling has fundamental effects on microglial distribution, morphology and function. Since P2Y12R is only expressed by microglia in the brain potentially allowing cell-specific targeting, studies are necessary to fully understand the consequences of P2Y12R-dependent interventions.

Additional Information

Supervisor:
Ádám Dénes
denes.adam@koki.mta.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4093

Start

12:32

End

12:35

Authors (legacy)

Balázs Pósfai1,2, Csaba Cserép1, Rebeka Fekete1, Krisztina Tóth1,2, Anett Dóra Schwarcz1, Katinka Ujvári1, Ádám Dénes1
1 Lendület Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest, Hungary
2 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary