PhD Scientific Days 2020

Molecular Sciences IV. Posters

Examination of the Putative „Regulatory Insertion” Region of ABCG2 Protein by Molecular Biological Methods

Előadó neve

Dr. Bartos, Zsuzsa

Előadó munkahelye

Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences Centre of Excellence

Előadó e-mail címe

bartos.zsuzsa.okla@gmail.com

Az előadás címe

Examination of the Putative „Regulatory Insertion” Region of ABCG2 Protein by Molecular Biological Methods

Szerző(k) neve és munkahelye

Zsuzsa Bartos1,2 and Tamás Hegedűs, PhD3
1 Doctoral School of Molecular Medicine, Pathobiochemistry Program
2 Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences Centre of Excellence
3 Department of Biophysics and Radiation Biology, Semmelweis University

Szekció

Molecular Sciences IV. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Összefoglaló szövege

The proper targeting to the correct membrane compartment is essential for the normal function of the urate and xenobiotic transporter ABCG2. Frequently, mutations or polymorphisms affect the cellular routing of the protein rather than its transport function. Previously, deletion of a regulatory insertion (RI) loop in the CFTR (ABCC7) was shown to increase the stability of this ABC protein. We identified a similar RI region in the ABCG2 protein using in silico methods. In order to examine the effect of removing this loop on the cellular localization and stability of ABCG2, we generated several deletion variants of the RI region. Protein level and maturation of these ABCG2 variants were determined by Western blotting and their cellular localization were studied by confocal microscopy. Our results show that the deletion of the “regulatory insertion” region does not stabilize the ABCG2 protein. Instead, RI plays a potential role in substrate recognition supported by in silico approaches.

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

5135

Start

12:58

End

13:01

Authors (legacy)

Zsuzsa Bartos1,2 and Tamás Hegedűs, PhD3
1 Doctoral School of Molecular Medicine, Pathobiochemistry Program
2 Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences Centre of Excellence
3 Department of Biophysics and Radiation Biology, Semmelweis University