PhD Scientific Days 2021

MO_I_P: Molecular Sciences I. Posters

SLAP deficiency prevent collagen induced arthritis

Előadó neve

Ms. Királyhidi, Panna

Előadó munkahelye

Department of Genetics, Cell- and Immunobiology, Semmelweis University

Előadó telefonszáma

+36303582098

Előadó e-mail címe

kiralyhidi.panna@gmail.com

Az előadás címe

SLAP deficiency prevent collagen induced arthritis

Szerző(k) neve és munkahelye

Panna Királyhidi1, Noémi Badari1, Zoltán Pós1, Tamás Németh2,3, Attila Mócsai2, Edit Buzás1, György Nagy1,3

1 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest
2 Department of Physiology, Semmelweis University, Budapest
3 Department of Rheumatology and Clinical Immunology, Semmelweis University, Budapest

Bemutatás módja

Poszter

Szekció

Molecular Sciences I. Posters

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

Introduction: in accordance with our previous data TNF α modulates T cell activation during inflammatory processes by regulating the CD3 ζ-chain expression via a Src-like adaptor protein (SLAP)-dependent mechanism. SLAP levels of the CD4+ T lymphocytes isolated from patients with RA were 2-fold higher than that of the healthy donors’, leading to the accelerated proteasomal degradation of CD3 ζ-chain. These data suggest the crucial role of SLAP in arthritis.

Aims: here we investigated the role of SLAP in collagen-induced arthritis (CIA).

Methods: SLAP knockout or heterozygous C57BL/6J mice were immunized with II. type collagen and complete Freund's adjuvant (CFA). Arthritis severity scores were monitored twice weekly for 2 months (1 limb score: 0=no swelling; 4=severe swelling), functional studies were also performed.

Results: The activity of arthritis (average total severity score) after the immunization was as follows (heterozygous vs. SLAP KO) at 5th, 6th and 8th weeks following immunization: 0 vs. 5.92 (P = 0.002); 0 vs. 8.75 (P = 0.002); 0 vs. 11.92 (P = 0.002) respectively.

Conclusion: SLAP deficiency completely protects from CIA.

Funding: UNKP-20-3 New National Excellence Program of the Ministry for Innovation and Technology; OTKA/NKFIHO NN 111023; OTKA/NKFIHN K 131479.

Reference: B. Érsek et al., J. Immunol., vol. 189, no. 4, pp. 1602–10, Aug. 2012.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Prof. Dr. György Nagy

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6047

Start

10:51

End

11:54

Authors (legacy)

Panna Királyhidi1, Noémi Badari1, Zoltán Pós1, Tamás Németh2,3, Attila Mócsai2, Edit Buzás1, György Nagy1,3

1 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest
2 Department of Physiology, Semmelweis University, Budapest
3 Department of Rheumatology and Clinical Immunology, Semmelweis University, Budapest