PhD Scientific Days 2021

PH_II_P: Pharmaceutical Sciences II. Posters

Analysing the Effect of the Alpha-2 Adrenoceptor Agonist Clonidine on Indomethacin-Induced Small Bowel Damage in Rats

Előadó neve

Mr. Tóth, András

Előadó munkahelye

SE ÁOK, Farmakológiai és Farmakoterápiás Intézet, Budapest

Előadó telefonszáma

+36307010772

Előadó e-mail címe

toth.andras_sebestyen@med.semmelweis-univ.hu

Az előadás címe

Analysing the Effect of the Alpha-2 Adrenoceptor Agonist Clonidine on Indomethacin-Induced Small Bowel Damage in Rats

Szerző(k) neve és munkahelye

András Tóth1, Barbara Hutka1, Patrik Pálnok1, Krisztián Kovács2, Klára Gyires1, Zoltán Zádori1
1 SE ÁOK, Farmakológiai és Farmakoterápiás Intézet, Budapest
2 SE ÁOK, Laboratóriumi Medicina Intézet, Budapest

Bemutatás módja

Poszter

Szekció

Pharmaceutical Sciences II. Posters

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

Introduction: It is increasingly recognized that non-steroidal anti-inflammatory drugs (NSAIDs) induce significant damage to the small intestine, with the prevalence rate of mucosal breaks of around 50% in chronic users. The development of small intestinal injury does not depend on acid secretion, but, instead, on other factors, including enteric bacteria, bile acids, prostaglandin deficiency and topical damaging effects. Since there is no well-established prophylactic approach, there is an urgent need to discover agents, that could prevent NSAID enteropathy. Our previous studies have demonstrated that alpha-2 adrenoceptor agonists induce protective effect in the stomach by multiple mechanisms. However, little is known about the effect of these medications on NSAID enteropathy.
Aims: To assess the effects of the alpha-2 adrenoceptor agonist clonidine on NSAID-induced enteropathy in an animal model.
Methods: Male Wistar rats (180-220 g) were treated with clonidine (10 and 100 microgram/kg) or its vehicle (distilled water) per os twice daily for 3 days. NSAID enteropathy was induced by a single dose of indomethacin (20 mg/kg), given per os on day 2. On day 4, rats were euthanized and the severity of enteropathy was evaluated by measuring serum parameters and intestinal inflammatory markers.
Results: Indomethacin-induced enteropathy was associated with shortening of the small bowel, elevation of intestinal myeloperoxidase and interleukin 1β, and with severe blood loss, and serum protein loss. Clonidine treatment had no significant effect on any of the tested parameters.
Conclusion: Our results suggest that pharmacological stimulation of alpha-2 adrenoceptors cannot prevent NSAID-induced entropathy, despite its well-established gastroprotective effect. This may be due to the different pathogenesis of gastro- and enteropathy, but further studies with other alpha-2 adrenoceptor agonists are required to confirm the present findings.
Funding: OTKA 124878, and EFOP-3.6.3-VEKOP-16-2017-00009

University and Doctoral School

Semmelweis University, Doctoral School of Pharmaceutical Sciences

Supervisor

Zoltán Zádori

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6050

Start

13:14

End

13:17

Authors (legacy)

András Tóth1, Barbara Hutka1, Patrik Pálnok1, Krisztián Kovács2, Klára Gyires1, Zoltán Zádori1
1 SE ÁOK, Farmakológiai és Farmakoterápiás Intézet, Budapest
2 SE ÁOK, Laboratóriumi Medicina Intézet, Budapest