PhD Scientific Days 2021

PH_I_P: Pharmaceutical Sciences I. Posters

Investigating Hydrogen-Sulfide (H2S) Delivery Capacity and Cardioprotective Effects of a H2S Releasing Ibuprofen Derivative

Előadó neve

Ms. Vass, Virág

Előadó munkahelye

Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary

Előadó telefonszáma

06704321733

Előadó e-mail címe

vass.virag@pharm.unideb.hu

Az előadás címe

Investigating Hydrogen-Sulfide (H2S) Delivery Capacity and Cardioprotective Effects of a H2S Releasing Ibuprofen Derivative

Szerző(k) neve és munkahelye

Virág Vass1,2, Erzsébet Szabó1, Ilona Bereczki3, Nóra Debreczeni3,4, Anikó Borbás3, Pál Herczegh3, Árpád Tósaki1
1 Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary
2 Doctoral School of Pharmaceutical Sciences, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary
3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary.
4 Doctoral School of Chemistry, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary.

Bemutatás módja

Poszter

Szekció

Pharmaceutical Sciences I. Posters

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

Introduction: Hydrogen sulfide (H2S) has been generated by tissues in a small quantity for physiological regulatory processes. Earlier studies proved that H2S and ibuprofen have a beneficial effect against tissue damage, and H2S reduces side effects of non-steroid anti-inflammatory (NSAI) drugs.
Aims: In our study ischemia/reperfusion was generated in isolated rat hearts where we investigated the H2S delivery capacity and cardioprotective effects of a new H2S donor molecule (BM-88).
Methods: Hearts were excised, and perfused in Langendorff mode. BM-88 was administered for 10 min before or after 30 min ischemia, reperfusion was performed for 120 min. The dose effect of BM-88 was determined by gradually increasing the concentration. The released H2S was measured from coronary effluent using electrochemical sensor. The protective effect of BM-88 can be confirmed by the infarct sizes of hearts. Autophagic and apoptotic markers (LC3-I/II, p62, Beclin1) were detected by immunohistochemistry method.
Result: We find that added H2S can be taken up and utilized by heart until 10µmol/ml concentration. BM-88 supports a long lasting H2S delivery in the heart tissues. It is likely that a treatment-induced decrease in infarct area was observed in BM-88 exposed hearts.
Conclusion: Confirming the protective effect of our new molecule may open a possibility for the development of cardioprotective agents with less side effects.
Funding: The work is funded by GINOP-2.3.2-15-2016-00043, NKFIH-K-124719 and supported by the EFOP-3.6.1-16-2016-00022 project. The project is co-financed by the European Union and the European Social Fund.

University and Doctoral School

Debrecen University Faculty of Medicine, Doctoral School of Pharmaceutical Sciences

Supervisor

Árpád Tósaki

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6067

Start

10:30

End

10:33

Authors (legacy)

Virág Vass1,2, Erzsébet Szabó1, Ilona Bereczki3, Nóra Debreczeni3,4, Anikó Borbás3, Pál Herczegh3, Árpád Tósaki1
1 Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary
2 Doctoral School of Pharmaceutical Sciences, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary
3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary.
4 Doctoral School of Chemistry, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary.