PhD Scientific Days 2021

TT_III_L: Theoretical and Translational Medicine III. Lectures

Roles of Nitric Oxide and Prostanoid Mediators in the Adaptation of the Cerebrocortical Microcirculation

Előadó neve

Mr. Hricisák, László

Előadó munkahelye

Institute of Translational Medicine, Semmelweis University

Előadó telefonszáma

+36-20/544-2491

Előadó e-mail címe

hricisak.laszlo@med.semmelweis-univ.hu

Az előadás címe

Roles of Nitric Oxide and Prostanoid Mediators in the Adaptation of the Cerebrocortical Microcirculation

Szerző(k) neve és munkahelye

László Hricisák1, Éva Pál1, Gergely Tamás Izsa1, Max Delank1, Dorina Nagy1, Laura Simoes Dobrydnio1, Nikolett Jabronka1, Ágnes Fülöp1, Zoltán Benyó1
1Institute of Translational Medicine, Semmelweis University, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Theoretical and Translational Medicine III. Lectures

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: The understanding of cerebral autoregulation has become of notable importance due to the increased incidence of carotid artery stenosis in the elderly population which entails an increased risk of stroke, the 2nd most common cause of mortality worldwide. Multiple mechanisms are debated in the literature, including the role of nitric oxide synthases (NOS) and prostanoid mediators. As we found earlier endothelial NOS (eNOS), surprisingly, does not seem to play an important role in the autoregulation. Now we aimed to determine the relevance of the simultaneous lack of endothelial and neuronal nitric oxide synthases (eNOS and nNOS) and prostanoid mediators (PMs).
Aims and Methods: We aimed to analyze the combined lack of eNOS and nNOS (double knock-out mice) and the role of prostanoid mediators (1 mg/kg indomethacin, TPR knock-out mice) in cerebrovascular autoregulation by analyzing the changes of cerebrocortical blood flow (CoBF) with the high temporal and spatial resolution of laser-speckle imaging after reducing the cerebral perfusion pressure by unilateral (left) common carotid artery occlusion (CAO).
Results: In eNOS/nNOS double KO animals the acute CoBF reduction after CAO was unaltered, but the recovery of CoBF was worsened as compared to controls. Indomethacin treatment resulted in a faster recovery in the temporal region. In TPR-KO animals, the recovery of the CoBF was diminished.
Conclusion: These results indicate that (1) the combined lack of eNOS and nNOS impairs only the subacute phase of the recovery after unilateral CAO and (2) indomethacin treatment results in a faster recovery, probably by inhibiting the release of a vasoconstrictor prostanoid, which is not thromboxane A2.
Grant and financial support: EFOP-3.6.3-VEKOP-16-2017-00009, OTKA K-125174, OTKA K-112964, NVKP_16-1-2016-0042, ÚNKP-20-4-I-SE-19.

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Prof. Zoltán Benyó

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4809

Start

17:10

End

17:25

Authors (legacy)

László Hricisák1, Éva Pál1, Gergely Tamás Izsa1, Max Delank1, Dorina Nagy1, Laura Simoes Dobrydnio1, Nikolett Jabronka1, Ágnes Fülöp1, Zoltán Benyó1
1Institute of Translational Medicine, Semmelweis University, Budapest, Hungary