PhD Scientific Days 2022

Translational Medicine I. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Cyclophilin D inhibition as a potential mitochondrial therapy following associating liver partition and portal vein ligation for staged hepatectomy in a mouse knockout model

Előadó neve

Dr. Daradics, Noemi

Előadó munkahelye

Semmelweis University; Department of Surgery, Transplantation and Interventional Gastroenterology; Hepato-Pancreatico-Biliary (HPB) Surgical Research Center Hungary; Budapest, Hungary

Előadó telefonszáma

206701424

Előadó e-mail címe

daradics.noemi@gmail.com

Az előadás címe

Cyclophilin D inhibition as a potential mitochondrial therapy following associating liver partition and portal vein ligation for staged hepatectomy in a mouse knockout model

Szerző(k) neve és munkahelye

Noemi Daradics1, Gergo Horvath2, Laszlo Tretter2, Agnes Paal3, Andras Fulop1, Andras Budai3, Attila Szijarto1
1 – Semmelweis University; Department of Surgery, Transplantation and Interventional Gastroenterology; Hepato-Pancreatico-Biliary (HPB) Surgical Research Center Hungary; Budapest, Hungary
2 – Semmelweis University; Department of Medical Biochemistry, Budapest, Hungary
3 – Semmelweis University; 2nd Department of Pathology; Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Translational Medicine I. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Aim: Inhibition of cyclophilins has been already proposed to be efficient as a mitochondrial therapy in liver diseases. To investigate the effect of Cyclophilin D (CypD) inhibition on mitochondrial function, biogenesis and liver regeneration following Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS) a CypD knockout (KO) mice model was created.
Methods: Male wild type (WT) (n=30) and CypD KO (n=30) mice underwent ALPPS procedure. Animals were terminated pre-operatively and 24, 48, 72 or 168 h after the operation. Mitochondrial functional studies and proteomic analysis of mitochondria were performed. Regeneration rate along with mitotic activity were assessed.
Results: The CypD KO group displayed improved mitochondrial function, as both ATP production (P < 0.001) and oxygen consumption (P < 0.05) were increased compared to the WT group. The level of mitochondrial biogenesis coordinator peroxisome proliferator-activated receptor γ co-activator 1-α (PGC1-α) was also elevated in the CypD KO group (P < 0.001), which resulted in the induction of the mitochondrial oxidative phosphorylation system. Liver growth was increased in the CypD KO group compared to the WT group (P < 0.001).
Conclusions: Our study demonstrates the effect of CypD inhibition on the mitochondrial vulnerability following ALPPS. Based on our results we propose that CypD inhibition should be further investigated as a possible mitochondrial therapy following ALPPS.

University and Doctoral School

Semmelweis University, Károly Rácz Doctoral School of Clinical Medicine

Supervisor

Prof. Dr. Szijártó Attila

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

5973

Start

11:15

End

11:20

Authors (legacy)

Noemi Daradics1, Gergo Horvath2, Laszlo Tretter2, Agnes Paal3, Andras Fulop1, Andras Budai3, Attila Szijarto1
1 – Semmelweis University; Department of Surgery, Transplantation and Interventional Gastroenterology; Hepato-Pancreatico-Biliary (HPB) Surgical Research Center Hungary; Budapest, Hungary
2 – Semmelweis University; Department of Medical Biochemistry, Budapest, Hungary
3 – Semmelweis University; 2nd Department of Pathology; Budapest, Hungary