PhD Scientific Days 2022

Neurosciences I. (Poster discussion will take place in the Aula during the Coffee Break)

The Role of Posterior Intralaminar Thalamic Neurons in Aggressive Behavior

Előadó neve

Láng, Tamás

Előadó munkahelye

Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University

Előadó telefonszáma

+36 30 367 9353

Előadó e-mail címe

lang.tamas98@gmail.com

Az előadás címe

The Role of Posterior Intralaminar Thalamic Neurons in Aggressive Behavior

Szerző(k) neve és munkahelye

Tamás Láng1, Dávid Keller1, Arpád Dobolyi1,2
1 Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest 1094, Hungary
2 MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Hungarian Academy of Sciences, Eötvös Loránd Research Network, and Eötvös Loránd University, Budapest, 1117, Hungary

Bemutatás módja

Szóbeli

Szekció

Neurosciences I. (Poster discussion will take place in the Aula during the Coffee Break)

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

In a previous study, we established the activation of the posterior intralaminar thalamic (PIL) neurons during social interactions between adult female rats. In this study we focused on the role of PIL in intermale aggressive behavior.
For manipulation of PIL neurons, adeno-associated virus was injected into the PIL using stereotaxic apparatus. The virus expressed mCherry and a DREADD in the infected cells. We used excitatory and inhibitory DREADDs, which were activated by clozapine-N-oxide (CNO). Behavioral tests were recorded during the chemogenetic manipulation. After perfusion of the animals, we performed histological analysis. We identified the brain areas activated by aggressive behavior using c-Fos method. We found neuronal activation in the ILC, MPOA and in the lateral septum upon aggressive behavior.
To induce aggression, the animals were separated at an early age. The behavioral tests were performed at the age 5 month. On the first day of the experiment, vehicle was injected to the animal. We performed aggressive behavioral test, where an unfamiliar intruder was placed in the subject animal’s cage resulting in aggressive response. On the second day, the same test was repeated starting 1,5 hours after CNO administration. Chemogenetic stimulation of PIL neurons significantly decreased aggression and increased the duration of positive valance contact, meanwhile inhibiting the PIL resulted in the increase of aggression and decreased the duration of positive valance contact. On the third day, we repeated the vehicle injection.
Based on these results, PIL neurons may participate in the regulation of aggressive behavior conveying sensory inputs from the conspecific to higher brain areas.

Grant support: NKFIH-4300-1/2017-NKP_17-00002, OTKA K1342221, EFOP-3.6.3-VEKOP-16-2017-00009

University and Doctoral School

Semmelweis University, János Szentágothai Doctoral School of Neurosciences

Supervisor

Prof. Árpád Dobolyi

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6815

Start

11:45

End

12:00

Authors (legacy)

Tamás Láng1, Dávid Keller1, Arpád Dobolyi1,2
1 Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest 1094, Hungary
2 MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Hungarian Academy of Sciences, Eötvös Loránd Research Network, and Eötvös Loránd University, Budapest, 1117, Hungary