PhD Scientific Days 2022

Molecular Sciences III. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Diverse effect of neutrophil granulocyte derived extracellular vesicles uptake on viability and functions of monocyte and macrophage cells

Előadó neve

Dr. Szeifert, Viktória

Előadó munkahelye

Department of Physiology

Előadó telefonszáma

+36304552299

Előadó e-mail címe

viktoriaszeifert@gmail.com

Az előadás címe

Diverse effect of neutrophil granulocyte derived extracellular vesicles uptake on viability and functions of monocyte and macrophage cells

Szerző(k) neve és munkahelye

1Viktória Szeifert, 1Nóra Borsos, 1Kirstóf Molnár, 1Mátka Nagy, 1Ákos M. Lőrincz
1Semmelweis University, Department of Physiology, Budapest

Bemutatás módja

Poszter

Szekció

Molecular Sciences III. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

Introduction: Previously, our group characterized distinct populations of extracellular vesicle (EV) released from neutrophilic granulocytes: EV formed spontaneously (spEV), during apoptosis (apoEV) and upon activation with opsonized particles (aEV). We observed diverse effect of these EV populations on resting neutrophils: aEV exerts pro-inflammatory, while spEV and apoEV show rather anti-inflammatory effect.
Aims: In the present work, our aim was to investigate the effect of the previously characterized neutrophil EVs on monocyte/macrophage cells.
Methods: We isolated neutrophils and monocytes from peripheral human blood. We collected the produced EVs after 20 minutes-long stimulation by two-step centrifugation and filtration. We normalized the EV number to their protein content determined by Bradford assay. We treated the monocytes with the EVs and measured their superoxide (ROS) production by lucigenin assay, the phagocytosis of S. aureus by flow cytometry and the cytokine production by sandwich ELISA. We also followed the viability of the cells after application of the EV samples for 48 hours by flow cytometry. We examined the differentiation of macrophages from stimulated monocytes via M-CSF with or without EV treatment on the 7. day, by flow cytometry.
Result: The PMN EV treatment did not alter the maximal ROS production of the monocytes, but the spEV delayed the opsonized Zymosan induced ROS production of the cells. The percentage of the phagocytosing monocytes decreased after the apoEV pre-treatment, but the phagocytic capacity was not affected. The spEV and apoEV increased the viability of the monocytes and also the viability and differentiation of the macrophages based on our first experiments. The TNF-α production of the monocytes increased by spEV pre-treatment and oZ-EV increased the TNF-α release in an even higher amount.
Conclusion: These results emphasize the diverse effect of EVs on other immune cells. These effects depend both on the state of the producing and the target cells.
Funding: OTKA FK 137770, EFOP-3.6.3-VEKOP-16-2017-00009, ÚNKP-21-3 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund, and NKFIH K119236.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Ákos Márton Lőrincz, MD, PhD

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4081

Start

12:45

End

12:50

Authors (legacy)

1Viktória Szeifert, 1Nóra Borsos, 1Kirstóf Molnár, 1Mátka Nagy, 1Ákos M. Lőrincz
1Semmelweis University, Department of Physiology, Budapest