PhD Scientific Days 2022

Pathology and Oncology II. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Analysis of DNA methylation through the development of colorectal adenocarcinoma

Előadó neve

Müller, Dalma

Előadó munkahelye

Semmelweis University

Előadó telefonszáma

702132172

Előadó e-mail címe

dalma.muller2@gmail.com

Az előadás címe

Analysis of DNA methylation through the development of colorectal adenocarcinoma

Szerző(k) neve és munkahelye

Müller Dalma1,2, Győrffy Balázs1,2
1 TTK Oncology Biomarker Research Group, Budapest, Hungary
2 Semmelweis University, Department of Bioinformatics, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Pathology and Oncology II. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

English

Preferred session

Pathology and Oncology

Összefoglaló szövege

Introduction. Alternations in the methylation pattern during tumorigenesis have been recognized decades ago and the identification of informative methylation patterns continues to be the focus of multiple cancer studies. Genome-wide methylation studies advance the understanding of colorectal adenocarcinoma progression and the identification of novel biomarkers.
Aim. Our goal was to assemble an integrated database containing normal colon, colon adenoma and adenocarcinoma tissue Illumina HumanMethylation450K data and to analyze the methylation pattern differences between different groups, especially the ones involved in normal-adenoma and adenoma-adenocarcinoma transition.
Methods. Data were downloaded from the GEO (Gene Expression Omnibus) database. Raw idat files were processed in R using the minfi and the wateRmelon library. To identify differentially methylated regions, gene level analysis was performed. The different regions were compared using Kruskal-Wallis-test. Significantly different regions were ranked based on beta value differences. Significantly different regions were ranked based on beta value differences.
Results. As a result, a database based on 19 studies containing 2,279 normal, adenoma, adenocarcinoma, and metastatic tissues data was established. In the gene promoter, significant hypermethylation of 0.2 ∆β was present when normal and adenoma, but not when adenoma and adenocarcinoma tissues were compared. In contrast, hypomethylation does seem to happen at a similar rate during normal-adenoma and adenoma-adenocarcinoma transitions.
Conclusion. We assembled a sizeable database containing colorectal samples with genome-wide methylation data and established a pipeline for data processing. Our results confirm that changes in DNA-methylation are early events of colorectal cancer development. Our database could be a useful starting point for biomarker discovery. To enable comfortable examination of the database we also aim to create a web-based interactive platform.
Funding: The research was financed by the FIEK_16-1-2016-0005, 2018-2.1.17-TET-KR-00001, and 2020-1.1.6-JÖVŐ-2021-00013 grants and by the Higher Education Institutional Excellence Programme (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary, within the framework of the Bionic thematic programme of the Semmelweis University.

University and Doctoral School

Semmelweis University, Doctoral School of Pathological Sciences

Supervisor

Balázs Győrffy MD PhD DSc

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6817

Start

11:15

End

11:20

Authors (legacy)

Müller Dalma1,2, Győrffy Balázs1,2
1 TTK Oncology Biomarker Research Group, Budapest, Hungary
2 Semmelweis University, Department of Bioinformatics, Budapest, Hungary