PhD Scientific Days 2022
Budapest, 6-7 July 2022
Clinical Medicine VII. (Poster discussion will take place in the Aula during the Coffee Break)
Immunohistochemical Study of the PD-1/PD-L1 Pathway in Cutaneous Lupus Erythematosus
Előadó neve
Dr. Király, Zsófia
Előadó munkahelye
Department of Dermatology, Venereology and Dermatooncology, Semmelweis University
Előadó telefonszáma
+36303851811
Előadó e-mail címe
zsofiakiraly00@gmail.com
Az előadás címe
Immunohistochemical Study of the PD-1/PD-L1 Pathway in Cutaneous Lupus Erythematosus
Szerző(k) neve és munkahelye
Zsófia Király 1, Ágota Szepesi 2, Anna Sebestyén 2, Enikő Kuroli 1,2, Fanni Rencz 3, Béla Tóth1, Laura Bokor 1, József Szakonyi 1, Márta Medvecz 1, Bernadett Hidvégi 1
1 Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 41 Mária Street, Budapest, H-1085, Hungary
2 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, 26 Üllői Street, Budapest, H-1085, Hungary
3 Department of Health Economics, Corvinus University of Budapest, Fővám Square 8., H-1093 Budapest, Hungary
Bemutatás módja
Szóbeli
Szekció
Clinical Medicine VII. (Poster discussion will take place in the Aula during the Coffee Break)
Language of the presentation
English
Preferred session
Clinical Medicine
Összefoglaló szövege
Introduction
The pathomechanism of various autoimmune diseases is known to be associated with the altered function of programmed cell death 1/programmed cell death ligand 1 (PD-1/PD-L1) axis.
Aims
We aimed to investigate the role of this pathway and inflammatory cell markers in subtypes of cutaneous lupus erythematosus (CLE): discoid lupus erythematosus (DLE), subacute CLE (SCLE) and toxic epidermal necrolysis (TEN)-like lupus, a hyperacute form of acute CLE (ACLE).
Method
Ten skin biopsy samples from 9 patients were analysed with immunohistochemistry regarding the following markers: CD3, CD4, CD8, Granzyme B, CD123, CD163, PD-1, PD-L1. Our group consisted of 4 SCLE (2 idiopathic and 2 PD-1 inhibitor-induced), 4 DLE and 1 TEN-like lupus cases. From the latter patient, two consecutive biopsies were obtained 1 week apart. Marker expression patterns were compared through descriptive analysis.
Results
Higher median keratinocyte (KC) PD-L1 expression was observed in the SCLE group compared to the DLE group (65% and 5%, respectively). Medians of dermal CD4, Granzyme B, PD-1 positive cell numbers and GB+/CD8+ ratio were higher in the DLE group than in the SCLE group. The consecutive samples of the TEN-like lupus patient showed an increase by time in the number of infiltrating Granzyme B+ cytotoxic T-cells and KC PD-L1 expression (from 22 to 43 and 30% to 70%, respectively).
Conclusion
High KC PD-L1 expression might serve as a defence mechanism in acute skin reactions like TEN-like lupus and SCLE. In case of DLE low KC PD-L1 expression may contribute to the chronic course of the disease.
Funding
This work was supported by grants from the EFOP-3.6.3-VEKOP-16-2017-00009.
University and Doctoral School
Semmelweis University, Károly Rácz Doctoral School of Clinical Medicine
Supervisor
Dr. Bernadett Hidvégi, PhD
Publication of my abstract
I give consent to the publication of my abstract on the website of the congress.
Kind
Szabad
Status
elfogadva
Accepted presentation method
szóbeli
Előadás fájl jóváhagyás
nem rendelkezett róla
Előadó
6011
Start
11:30
End
11:45
Authors (legacy)
Zsófia Király 1, Ágota Szepesi 2, Anna Sebestyén 2, Enikő Kuroli 1,2, Fanni Rencz 3, Béla Tóth1, Laura Bokor 1, József Szakonyi 1, Márta Medvecz 1, Bernadett Hidvégi 1
1 Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 41 Mária Street, Budapest, H-1085, Hungary
2 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, 26 Üllői Street, Budapest, H-1085, Hungary
3 Department of Health Economics, Corvinus University of Budapest, Fővám Square 8., H-1093 Budapest, Hungary