PhD Scientific Days 2022

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

Signature of miRNAs in human chronic kidney disease and animal models - a meta-analysis of profiling studies

Előadó neve

Dr. Garmaa, Gantsetseg

Előadó munkahelye

Institue of Translational Medicine

Előadó telefonszáma

+36-205720871

Előadó e-mail címe

gantsetseg.garmaa@gmail.com

Az előadás címe

Signature of miRNAs in human chronic kidney disease and animal models - a meta-analysis of profiling studies

Szerző(k) neve és munkahelye

Garmaa Gantsetseg1,2, Stefania Bunduc2,3,4,6,7, Hegyi Péter2,3,4,5, Csupor Dezső1,3,8, Dembrovszky Fanni2,3, Peter Fehervari 2,9, Fanni Meznerics2,10, Ailar Nasirzadeh1, Gábor Kökény1
1 Institute of Translational Medicine, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary
2 Center for Translational Medicine, Semmelweis University, 1085 Budapest, Üllői út 26, Hungary
3 Institute for Translational Medicine, Medical School, University of Pécs, 7624 Pécs, Szigeti út 12, Hungary
4 Division of Pancreatic Diseases, Heart and Vascular Center, Semmelweis University, 1085 Budapest, Baross út 22-24, Hungary
5 János Szentágothai Research Center, University of Pécs, 7624 Pécs, Szigeti út 12, Hungary
6 Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Dionisie Lupu street 37, Romania
7 Fundeni Clinical Institute, 022328 Bucharest, Fundeni street 258, Romania
8 Institute of Clinical Pharmacy, University of Szeged, 6725 Szeged, Szikra utca 8, Hungary
9 University of Veterinary Medicine, Budapest Dept. Of Biomathematics and Informatics István st. 2, Budapest, Hungary H-1078
10 Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 1085 Budapest, Mária ut 41, Hungary

Bemutatás módja

Szóbeli

Szekció

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Chronic kidney disease (CKD) represents a major health burden worldwide. There is increasing proof of the role of microRNA (miR) in CKD. A synthesis of the available evidence will facilitate its translation to clinical practice. We performed a systematic review and meta-analysis of miR expression profiling studies in humans and animal models with CKD. The systematic search was performed in five databases from inception to October 17, 2021. The Robust Rank Aggregation method was used to identify the most dysregulated mature miRs. We retrieved the predicted and validated target genes for the identified miRs with the DIANA microT-CDS (v.5.0), TargetScan, and TarBase (v.7.0) tools, followed by KEGG pathway enrichment analysis. We identified 32 humans, and 13 rodent eligible studies (1374 and 202 cases, 888 and 202 controls, respectively). Nine miRs were significantly downregulated in human: four in urine (miR-133a-3p, miR-3180-3p, miR-181a-5p, miR-936), two in blood (miR-451a, miR-106b-5p), and three in kidney tissue (miR-486-3p, miR-486-5p, miR-451a). The most upregulated miRs in human were urinary miR-27a-3p, and miR-1260b in blood. The rodent studies reported upregulation of miR-20a-5p, miR-709, miR-429-3p, and downregulation of miR-21a-5p, miR-223-3p, miR-342-3p, miR-214-3p, and miR-199a-5p in kidney. In enrichment analysis, thyroid hormone signaling, Hippo signaling, Wnt signaling, and central carbon metabolism in cancer and lysine degradation pathways were most affected by upregulated miRs. The downregulated miRs affected lysine degradation, Hippo signaling, proteoglycans in cancer, and adherent junction pathways. The miR signature and molecular pathways revealed in our meta-analysis are promising candidates for further detailed CKD studies and diagnostic markers.

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Gábor Kökény

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6476

Start

09:00

End

09:15

Authors (legacy)

Garmaa Gantsetseg1,2, Stefania Bunduc2,3,4,6,7, Hegyi Péter2,3,4,5, Csupor Dezső1,3,8, Dembrovszky Fanni2,3, Peter Fehervari 2,9, Fanni Meznerics2,10, Ailar Nasirzadeh1, Gábor Kökény1
1 Institute of Translational Medicine, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary
2 Center for Translational Medicine, Semmelweis University, 1085 Budapest, Üllői út 26, Hungary
3 Institute for Translational Medicine, Medical School, University of Pécs, 7624 Pécs, Szigeti út 12, Hungary
4 Division of Pancreatic Diseases, Heart and Vascular Center, Semmelweis University, 1085 Budapest, Baross út 22-24, Hungary
5 János Szentágothai Research Center, University of Pécs, 7624 Pécs, Szigeti út 12, Hungary
6 Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Dionisie Lupu street 37, Romania
7 Fundeni Clinical Institute, 022328 Bucharest, Fundeni street 258, Romania
8 Institute of Clinical Pharmacy, University of Szeged, 6725 Szeged, Szikra utca 8, Hungary
9 University of Veterinary Medicine, Budapest Dept. Of Biomathematics and Informatics István st. 2, Budapest, Hungary H-1078
10 Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 1085 Budapest, Mária ut 41, Hungary