PhD Scientific Days 2022

Pathology and Oncology III. (Poster discussion will take place in the Aula during the Coffee Break)

Tumor-Stroma Ratio in Breast Cancer: Comparison of Findings in Core Biopsies and Resection Specimens

Előadó neve

Karancsi, Zsófia

Előadó munkahelye

Department of Pathology, Forensic and Insurance Medicine

Előadó telefonszáma

+36303147534

Előadó e-mail címe

zsofi.karancsi@gmail.com

Az előadás címe

Tumor-Stroma Ratio in Breast Cancer: Comparison of Findings in Core Biopsies and Resection Specimens

Szerző(k) neve és munkahelye

Karancsi Zsófia1, Sophie Hagenaars2
1 Department of Pathology, Forensic and Insurance Medicine, Budapest
2 Leiden University Medical Centre, Leiden

Bemutatás módja

Szóbeli

Szekció

Pathology and Oncology III. (Poster discussion will take place in the Aula during the Coffee Break)

Language of the presentation

English

Preferred session

Pathology and Oncology

Összefoglaló szövege

Introduction: Tumor-stroma ratio (TSR) is considered an important prognostic and predictive factor in several tumor types. In our previous study we found that the TSR of the core biopsy can be used as a predictive factor in planning neoadjuvant chemotherapy.
Aims: We aim to analyse whether TSR measured on breast cancer core biopsy is representative of the whole tumor and how is it associated with prognosis. Only few scientifically substantiated data describe the scoring method in core biopsies compared with resections specimens, thus we analysed different scoring methods and their reproducibility and aimed to find the most suitable method.
Methods: 178 core biopsies and the corresponding resection specimens from 174 patients treated with primary surgical therapy were investigated. Patients with median age of 63.5 year (28-90) were diagnosed between 2010-2021. Clinicopathological data were obtained from the medical database of Semmelweis University (Permission SE RKEB: 244/2019). Recurrence-free survival (RFS) was defined as the time from surgery to the occurrence of any breast cancer related event. Hematoxylin-eosin-stained, digital slides were analyzed based on the methodology recommended by Mesker et al., at x100 and x200 magnification by two trained scientists (ZsK-Budapest and SH-Leiden University). On the most stroma rich area TSR was visually determined by tenfold percentage steps and the cases were divided into two groups: ≤50% stromal area as stroma low (SL) and >50% stromal area as stroma high (SH).
Results: The interobserver variability of TSR was low (core biopsy, x100: κ =0.91, x200: κ =0.79, resection specimen: x100: κ =0.89, x200: κ=0.73). Looking at the similarities of the scores in biopsy and resection specimen, the highest correlation was found using x100 magnification. Discrepancies were more frequent if the biopsy contained small amount of tumor or if the resection specimen had only one stroma high area. There were 21 recurrencies during the follow-up period. Among 162 (91%) hormone receptor positive (HR+) tumors stroma high cases were associated with significantly shorter RFS (p=0.046 at 60% cut-off point).
Conclusions: Based on our findings scoring TSR on core biopsies is reproducible and mostly representative of the whole tumor and can be used as a prognostic factor in HR+ cases.
Funding: 3.6.3-VEKOP-16-2017-00009

University and Doctoral School

Semmelweis University, Doctoral School of Pathological Sciences

Supervisor

Prof. Kulka Janina, Dr. Tőkés Anna-Mária

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6893

Start

09:30

End

09:45

Authors (legacy)

Karancsi Zsófia1, Sophie Hagenaars2
1 Department of Pathology, Forensic and Insurance Medicine, Budapest
2 Leiden University Medical Centre, Leiden