PhD Scientific Days 2022

Pharmaceutical Sciences I.

Development of liposomal corticosteroids

Előadó neve

Dr. Budavári, Bálint

Előadó munkahelye

Department of Biophysics and Radiation Biology

Előadó telefonszáma

+36309562776

Előadó e-mail címe

balint.budavari@gmail.com

Az előadás címe

Development of liposomal corticosteroids

Szerző(k) neve és munkahelye

Bálint Budavári 1 , Balázs Gábor Pinke 2 , Kolos Molnár 2 , Dominik Sziklai 1 , István Voszka 1 ,
Angéla Jedlovszky-Hajdú 1 , Krisztina S. Nagy 1
1 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
2 Department of Polymer Engineering, Budapest University of Technology and Economics

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences I.

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

Introduction: Corticosteroid-loaded liposomes are nano-scale drug-delivery systems. This
approach has several benefits in the anti-inflammatory therapy in terms of biopharmaceutical
parameters like adsorption, biodistribution and controlled release of the drug.
Aims: Our goals were the following: 1) to reveal the effect of the incorporated steroid
molecules on the phase-transition temperature (T c ) of liposomes; 2) to find the best
composition of phospholipids keeping the T c of liposomes in the range of inflamed tissue
temperature; 3) to visualize the liposomes.
Method: Three different kinds of lipids: dipalmitoyl-phosphatidylcholine (DPPC), distearoyl-
phosphatidylcholine (DSPC), and dimyristoyl-phosphatidylcholine (DMPC) were used in 2
combinations to prepare prednisolone-loaded liposomes. Small unilamellar liposomes were
gained by thin layer hydration followed by extrusion. The particle size-distribution was
measured by dynamic light scattering (DLS) for 1 year. Differential scanning calorimetry
(DSC) was used to determine the exact T c values. The liposomes were visualized by atomic
force microscopy (AFM).
Results: The drug-loaded liposomes showed narrow particle size distribution around 100 nm
of hydrodynamic diameter througout the 1-year long observation period for each composition.
The DSC results revealed 40-41 °C of T c for drug-free liposomes and no significant influence
of prednisolone. The AFM pictures proved the predicted-size of liposomes.
Conclusions: We could successfully create prednisolone-loaded liposomes with 100 nm in
diameter, which were stable at least for 1 year at 25°C. The calculated and measured T c values
are almost the same with or without added drug.
Funding: Semmelweis 250+ Excellence PhD Scholarship, EFOP-3.6.3-VEKOP-16-2017-
00009, NKFI FK 124147, TKP2021-EGA-23

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Dr. Krisztina S. Nagy

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6089

Start

15:30

End

15:45

Authors (legacy)

Bálint Budavári 1 , Balázs Gábor Pinke 2 , Kolos Molnár 2 , Dominik Sziklai 1 , István Voszka 1 ,
Angéla Jedlovszky-Hajdú 1 , Krisztina S. Nagy 1
1 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
2 Department of Polymer Engineering, Budapest University of Technology and Economics