PhD Scientific Days 2022

Clinical Medicine VII. (Poster discussion will take place in the Aula during the Coffee Break)

Evaluation of an Amino Acid Based Solid Scaffold in Bone Defects Using Rat Calvaria Model

Előadó neve

Dr. Czumbel, László Márk

Előadó munkahelye

Department of Oral Biology

Előadó telefonszáma

+36703969300

Előadó e-mail címe

markczumbel@hotmail.com

Az előadás címe

Evaluation of an Amino Acid Based Solid Scaffold in Bone Defects Using Rat Calvaria Model

Szerző(k) neve és munkahelye

László Márk Czumbel1, Róbert Rácz1, Dávid Pammer2, Sándor Farkasdi1, Dániel Csete3, Dávid Juriga4, Angéla Jedlovszky-Hajdú4, Gábor Gerber5, Gábor Varga1

1Department of Oral biology, Semmelweis University, Budapest
2Department of Materials Science and Engineering, Budapest University of Technology and Economics, Budapest
3Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest
4Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
5Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest

Bemutatás módja

Poszter

Szekció

Clinical Medicine VII. (Poster discussion will take place in the Aula during the Coffee Break)

Language of the presentation

English

Preferred session

Dentistry

Összefoglaló szövege

Introduction: In orthopedics, head and neck surgery, and also in dentistry bone grafts play an important role to reconstruct bone defects and give support for the covering soft tissues. For larger bone deficiencies, the “gold standard” therapy is autogenous bone grafting. However, this procedure can have significant drawbacks: formation of hematoma and higher risk of infection. Thus, there is a great need for solid scaffolds that promote and guide the healing of bone defects. Recently we have developed polyaspartic acid (PASP)-based hydrogels, which can serve as a candidate for bone graft and characterized their biocompatibility in vitro. However, currently no preclinical data are available for PASP-based hydrogels affecting bone regeneration.
Aims: Thus, we aimed to investigate the effects of a PASP-based hydrogel on bone growth in rat calvaria bone defect model.
Methods: In our study we used male Wistar rats. Ethical permission number: PE/EA/917-7/2020. We created two identical bi-cortical defects (6 mm in diameter) in to the calvaria of each animal. PASP-gel based solid disks were placed into one of the defect while the other one was left empty. We sutured the periosteum and the skin per primam after placing the PASP gel. Biological changes in the bone were evaluated using micro-CT analysis and histologic methods. We used t-test for statistical analysis.
Results: After 2 months of healing there were no significant differences between the two groups in bone volume to tissue volume ratio (BV/TV). BV/TV: 16.80±3.06, n=6, and 10.74±2.44, n=6 for the control and PASP groups, respectively (p>0.05). Additionally, CT images revealed no signs of inflammation or further bone resorption around the disks. Moreover, analysis reveal that bone ingrowths started in the empty defects, while in the PASP-filled defects bone grew on the top of the PASP-gels disks.
Conclusion: Based on the CT images PASP hydrogel is a biocompatible material. Additionally, it can guide bone deposition, however, no significant add-on effect was detected on the rate of new bone production compared to the untreated defects.
Funding: The project was supported by the Hungarian Ministry of Human Resources, the New Hungarian Program of National Excellence (ÚNKP-21-4-I).

University and Doctoral School

Semmelweis University, Károly Rácz Doctoral School of Clinical Medicine

Supervisor

Prof. Dr. Varga Gábor

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2731

Start

13:40

End

13:45

Authors (legacy)

László Márk Czumbel1, Róbert Rácz1, Dávid Pammer2, Sándor Farkasdi1, Dániel Csete3, Dávid Juriga4, Angéla Jedlovszky-Hajdú4, Gábor Gerber5, Gábor Varga1

1Department of Oral biology, Semmelweis University, Budapest
2Department of Materials Science and Engineering, Budapest University of Technology and Economics, Budapest
3Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest
4Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
5Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest