PhD Scientific Days 2022

Pathology and Oncology II. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Relationship between Tumor Infiltrating Lymphocytes and Inflammasoma Activity in Pancreatic Neoplasms

Előadó neve

Dr. Németh, Kristóf

Előadó munkahelye

Department of Pathology, Forensics and Insurance Medicine- Semmelweis University

Előadó telefonszáma

+36304026645

Előadó e-mail címe

nemkikko@gmail.com

Az előadás címe

Relationship between Tumor Infiltrating Lymphocytes and Inflammasoma Activity in Pancreatic Neoplasms

Szerző(k) neve és munkahelye

Kristóf Németh M.D., Eszter Mezei
1 Department of Pathology, Forensics and Insurance Medicine/Semmelweis University, Budapest
2 Department of Pathology, Forensics and Insurance Medicine/Semmelweis University, Budapest

Bemutatás módja

Szóbeli

Szekció

Pathology and Oncology II. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

Hungarian

Preferred session

Pathology and Oncology

Összefoglaló szövege

Introduction: Intraductal papillary mucinous neoplasia (IPMN) is the most common cystic lesion of the pancreas. The clinical significance of it is given by its relative asymptomaticity and high malignant potential. Recently, the effect of the immunosuppressive stromal microenvironment in tumor progression became known in different types of tumors. This special microenvironment can be caused by the NLRP3 inflammasome activation and tumor-infiltrating lymphocytes. Aims: The aim of the present research is to determine the effect of the effector molecules in the inflammatory signaling pathway on survival and the oncopathological properties of IPMN. Methods: In our study, we examined histological specimens from patients diagnosed with IPMN at the Department of Pathology, Forensics and Insurance Medicine between 2008 and 2018 (n = 70). Tissue microarrays (TMA) were prepared from the samples and labeled by immunohistochemistry using antibodies to IL-1β, IL-18, IL-1Ra, caspase-1, and CD4, and sections were digitized and evaluated by H-Score and cell counting. Patient data were collected from the institute's internal database (MedRec), the electronic health record system of Semmelweis University, and the database of the Central Statistical Office. Results: Patient survival reduced in the presence of elevated protein expression of IL-1β (p = 0.05), IL-18 (p = 0.05), IL-1Ra (p = 0.000019). In invasive cases, we observed significantly higher IL-1β (p = 0.013) and a tendency to higher IL-18 and IL-1Ra expression. Lower IL-18 levels were measured in mixed ductal involvement (p = 0.043) and intestinal subtype (p = 0.03). Lower CD4+ cell infiltration was present with lymphovascular invasion (p = 0.03). Examining the correlation of these proteins, it can be said that the expression of IL-1β, IL-18, IL-1RA, as well as the lymphocyte density and the expression of IL-18 and caspase-1 showed a significant positive correlation. Conclusion: The differential expression of effector molecules in the inflammasome signaling pathway has prognostic and may have therapeutic value for the patients with IPMN. Determination of IL-1Ra expression can be used as a strong and sensitive negative prognostic factor.

University and Doctoral School

Semmelweis University, Doctoral School of Pathological Sciences

Supervisor

Budai András M.D.

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6906

Start

09:45

End

10:00

Authors (legacy)

Kristóf Németh M.D., Eszter Mezei
1 Department of Pathology, Forensics and Insurance Medicine/Semmelweis University, Budapest
2 Department of Pathology, Forensics and Insurance Medicine/Semmelweis University, Budapest