PhD Scientific Days 2022

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

Visualization of Organ-Specific Lymphatic Growth: A New Approach

Előadó neve

Christ, Carolin, MSc

Előadó munkahelye

Semmelweis University, Physiology Department

Előadó telefonszáma

+36707034970

Előadó e-mail címe

caro.christ90@gmail.com

Az előadás címe

Visualization of Organ-Specific Lymphatic Growth: A New Approach

Szerző(k) neve és munkahelye

Carolin Christ1, Zoltan Jakus1
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction:
Lymphatic vessels are important for the maintenance of healthy organs. Changes in lymphatic function or morphology are known to play a role in the development of lymphedema, atherosclerosis, high-blood pressure and other metabolic diseases. The pathophysiological role of lymphatic vessels is not fully understood yet, mainly due to the lack of visualization techniques.
Aim:
In this study we aimed to develop a new technique to visualize organ-specific lymphatic growth.
Materials and Methods:
To visualize organ-specific growth of lymphatics, different organs of CL57BL/6 and Prox-1GFP reporter mice were tissue cleared, followed by whole mount immunostainings. In parallel, we performed paraffin based histology, followed by immunostainings to show the difference between traditional visualization methods and our newly developed visualization technique. Flt4+/+ and Flt4kd/+ mice were used as a model for lymphatic alterations. Pictures were taken by upright, stereo and confocal microscopy.
Results:
We could demonstrate that tissue clearing, followed by whole mount immunostaining, is a very effective way to visualize organ-specific lymphatic growth, especially in comparison to older techniques such as simple immunostaining of paraffin sections. With this method we are able to visualize lymphatic vessels in 3D, show differences in the lymphatic morphology between organs and compare and quantify the lymphatics of different mouse models.
Conclusion:
Our results show that tissue clearing in combination with whole mount immunostaining is a great technique to visualize the organ-specific growth of lymphatic vessels. This new approach can help to clarify the role of lymphatic vessels in the pathology of various diseases.
Funding:
This work was supported by the National Research, Development and Innovation Office (NVKP_16-2016-1-0039, K 139165, TKP2021-EGA-29, TKP2021-EGA-24), the European Union and the Hungarian Government (VEKOP-2.3.2-16-2016-00002), Scientific and Innovative Research Fund of the Semmelweis University, and the Higher Education Institutional Excellence Program of the Ministry for Innovation and Technology in Hungary, within the framework of the Molecular Biology thematic program of the Semmelweis University.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Dr. Zoltán Jakus

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4128

Start

09:30

End

09:45

Authors (legacy)

Carolin Christ1, Zoltan Jakus1
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary