PhD Scientific Days 2022

Molecular Sciences IV.

Characterization of the Lymphatic Vasculature in Atherosclerosis

Előadó neve

Christ, Carolin, MSc

Előadó munkahelye

Semmelweis University, Physiology Department

Előadó telefonszáma

+36707034970

Előadó e-mail címe

caro.christ90@gmail.com

Az előadás címe

Characterization of the Lymphatic Vasculature in Atherosclerosis

Szerző(k) neve és munkahelye

Carolin Christ1, Zoltán Jakus1
1Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Molecular Sciences IV.

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

Introduction:
Lymphatic vessels are present in the arterial wall, but the role of these vessels is not fully understood yet. Recently it has been shown that lymphatic vessels participate in reverse cholesterol transport, suggesting a possible role in the development of atherosclerosis.
Aim:
In this study we aimed to characterize the morphology and growth of the lymphatic vasculature in atherosclerosis.
Materials and Methods:
To visualize lymphatic vessels in the arterial wall, whole aortas were tissue cleared, followed by immunostaining. In parallel, Ldlr -/- and ApoE-/- mice on chow or western diet were used to characterize the lymphatic vasculature in atherosclerosis by whole aorta stainings and paraffin-based histology followed by immunostainings. To see if lymphangiogenesis has an impact on the development of atherosclerosis, we investigated ApoE-/- mice with a conditional deletion of a lymphangiogenic factor.
Results:
We could demonstrate that lymphatic vessels are present in the adventitia of the aorta. Both Ldlr -/- and ApoE-/- mice developed severe atherosclerosis on western diet, indicating the largest plaques in the brachiocephalic artery and the aortic arch. Atherosclerotic mice showed an increased number of lymphatic vessels in the arterial wall as well as changes in the morphology of those vessels. Experiments with ApoE-/- mice with a deleted lymphangiogenic factor showed that female mice develop more calcification in the aortic arch.
Conclusion:
Our results suggest the possible role of the lymphatic vasculature in the development of atherosclerosis. Defining the role of the lymphatic system in the pathogenesis of atherosclerosis may lead to the development of novel therapeutic approaches in the future.
Funding:
This work was supported by the National Research, Development and Innovation Office (NVKP_16-2016-1-0039, K 139165, TKP2021-EGA-29, TKP2021-EGA-24), the European Union and the Hungarian Government (VEKOP-2.3.2-16-2016-00002), Scientific and Innovative Research Fund of the Semmelweis University, and the Higher Education Institutional Excellence Program of the Ministry for Innovation and Technology in Hungary, within the framework of the Molecular Biology thematic program of the Semmelweis University.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Dr. Zoltán Jakus

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4128

Start

15:00

End

15:15

Authors (legacy)

Carolin Christ1, Zoltán Jakus1
1Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary