PhD Scientific Days 2022

Molecular Sciences IV.

CCBE1 regulates the development and prevents the age-dependent degradation of meningeal lymphatics

Előadó neve

Dr. Ocskay, Zsombor

Előadó munkahelye

Semmelweis University Department of Physiology

Előadó telefonszáma

06205802280

Előadó e-mail címe

zsombor.ocskay@med.semmelweis-univ.hu

Az előadás címe

CCBE1 regulates the development and prevents the age-dependent degradation of meningeal lymphatics

Szerző(k) neve és munkahelye

Zsombor Ocskay1, László Bálint1, Carolin Christ1, Zoltán Jakus1
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Molecular Sciences IV.

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

The mLVs (meningeal lymphatic vessels) develop postnatally and are degraded by aging. For this developmental program and maintenance, VEGFC/VEGFR3 signaling is essential.
Recent in vitro works have suggested that the collagen- and calcium-binding EGF domains 1 (CCBE1) protein promotes lymphogenic VEGFC maturing and binding.
We aimed to investigate the organ-specific role of the CCBE1 in developmental lymphangiogenesis and maintenance of lymphatic vessels during aging.
Lymphatic endothelial cell-specific immunostainings were used to characterize the meningeal lymphatic structures in wild-type and CCBE1 deficient mice at different ages, including young and old animals. The lymphatic function was monitored by injecting labeled macromolecules into the CNS, then assessing uptake and drainage to the cervical lymph nodes.
We have demonstrated that the CCBE1 inducible deletion impaired the postnatal development of the meningeal lymphatics and decreased macromolecule drainage from the CNS. We revealed that the structural integrity and density of mLV vessels gradually altered during aging. Surprisingly, in adult mice, the LVs showed regression after CCBE1 deletion.
Our results indicate the importance of lymphangiogenic mechanisms in developing and preventing the age-related degradation of meningeal lymphatics.
The degradation of meningeal lymphatic structures in aging may play an essential role in the pathophysiology of Alzheimer's disease.
Funding:
This work was supported by the National Research, Development and Innovation Office (NVKP_16-2016-1-0039, K 139165, TKP2021-EGA-29, TKP2021-EGA-24 to Z.J.), the European Union and the Hungarian Government (VEKOP-2.3.2-16-2016-00002 to Z.J. and EFOP-3.6.3-VEKOP-16-2017-00009 to O.Z.), and the Higher Education Institutional Excellence Program of the Ministry for Innovation and Technology in Hungary, within the framework of the Molecular Biology thematic program of the Semmelweis University.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Zoltán Jakus MD,PhD

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4805

Start

15:45

End

16:00

Authors (legacy)

Zsombor Ocskay1, László Bálint1, Carolin Christ1, Zoltán Jakus1
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary