PhD Scientific Days 2022

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

The Role of Microglia in the Regulation of Neuronal Activity

Előadó neve

Dr. Pósfai, Balázs

Előadó munkahelye

Institute of Experimental Medicine

Előadó telefonszáma

+36303313010

Előadó e-mail címe

posfai.balazs@koki.hu

Az előadás címe

The Role of Microglia in the Regulation of Neuronal Activity

Szerző(k) neve és munkahelye

Balázs Pósfai1, Andor Domonkos2, Csaba Cserép1, Ádám Dénes1
1 Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest
2 Laboratory of Thalamus Research, Institute of Experimental Medicine, Budapest

Bemutatás módja

Poszter

Szekció

Theoretical Medicine (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

Hungarian

Preferred session

Neurosciences

Összefoglaló szövege

Introduction: Microglia are the main immune cells of the brain with essential roles in pathophysiological processes, as well as in the maintenance of brain homeostasis. In recent years, microglia were shown to influence fundamental neuronal processes, like differentiation, survival or cell death, while microglial processes also regulate synaptic plasticity through direct contacts with synaptic profiles. We discovered a membrane-membrane contact between microglial processes and neuronal cell bodies that are present on the vast majority of cortical neurons and interneurons, and through which microglia are able to modulate neuronal mitochondrial activity and exert neuroprotection. However, the effect of microglia on neuronal and network activity is unknown.

Aims: The aim of our experiments is to better understand the role of microglia in regulating neuronal activity in the cerebral cortex and the hippocampus of mice, by comparing neuronal activity before and after selective microglia depletion.

Methods: To study microglia-neuron interactions at individual neurons, we have established intracranial EEG measurements in freely moving mice using 64-channel polytrodes (A1x64-Poly2-6mm-23s-160-H64_30mm, NeuroNexus). 4-5 months old C57BL/6 mice underwent surgical implantation of polytrodes, recording sites of which spanned from the primary somatosensory cortex to the str. radiatum of the dorsal hippocampal CA1 region. Mice were observed in three different environments: home cage, open field and linear track. Signals are acquired using an RHD2000 interface board (Intan Technologies). The movement of the animal is tracked with 6 high speed cameras (Flex13, OptiTrack), using an infrared marker-based approach. After control recordings, mice were fed a chow diet containing the CSF1 receptor antagonist, PLX5622 (Plexxikon Inc.) for 3 weeks to eliminate microglia from the brain, and recordings were repeated. Evaluation was carried out using custom-written MATLAB functions.

Results and conclusions: Our preliminary data show a selective effect of microglial depletion on the activity of fast-spiking interneurons. We are also preparing experiments with chemogenetic microglial manipulation.

Funding: Supported by the ÚNKP-21-4 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund.

University and Doctoral School

Semmelweis University, János Szentágothai Doctoral School of Neurosciences

Supervisor

Csaba Cserép MD PhD, Ádám Dénes PhD

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4093

Start

10:30

End

10:35

Authors (legacy)

Balázs Pósfai1, Andor Domonkos2, Csaba Cserép1, Ádám Dénes1
1 Laboratory of Neuroimmunology, Institute of Experimental Medicine, Budapest
2 Laboratory of Thalamus Research, Institute of Experimental Medicine, Budapest