PhD Scientific Days 2022

Pharmaceutical Sciences III. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Effect of a MicroRNA Mimic on Cardiac Gene Expression in Mice

Előadó neve

Dr. Makkos, András

Előadó munkahelye

Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

+36703707325

Előadó e-mail címe

makkos.andras@med.semmelweis-univ.hu

Az előadás címe

Effect of a MicroRNA Mimic on Cardiac Gene Expression in Mice

Szerző(k) neve és munkahelye

Bence Ágg1, Zoltán V. Varga1, Anikó Görbe1, Péter Ferdinandy1
1 Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences III. (Poster discussion will take place on the terrace of the room during the Coffee Break)

Language of the presentation

English

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

Background: Cardiovascular diseases are leading cause of death worldwide. Novel therapies in the field have great potential to alleviate the burden of disease in terms of both social economics and individual morbidity. MicroRNAs are great candidates of these novel approaches.
Aim: The objective of this study is to investigate the effects of a miRNA mimic on myocardial gene expression in a small animal model.
Methods: Single injection of 10 µg miRNA mimic in neutral lipid emulsion was injected intravenously into mice. Animals were sacrificed 1, 2, 4, 8 or 24 hours after treatment administration. At the time of termination, plasma and left ventricular myocardium tissue samples were collected. Total RNA was isolated from the samples, and complementary DNA library was prepared for further analysis. MiRNA and selected target mRNA expression levels were measured with qRT-PCR.
Results: 47 from 50 mice underwent successful tail vein injection and no acute side effects were observed. 1 hour after treatment, the expression of the miRNA was nearly 20-fold elevated in plasma and 8-10-fold elevated in the myocardium, however, this elevation decreased rapidly. The expression of 3 selected target mRNAs out of 5 was reduced 8 hours after injection.
Conclusions: The applied intravenous miRNA mimic was able to reach the myocardium and induce an elevation in miRNA level. The observed difference in the time frame of the induced miRNA and target mRNA changes suggests a shift in the development of changes in target gene expression. This finding can be of importance in future studies and drug development.

University and Doctoral School

Semmelweis University, Doctoral School of Pharmaceutical Sciences

Supervisor

Anikó Görbe, MD, PhD, Péter Ferdinandy, MD, PhD, DSc, MBA

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1293

Start

12:00

End

12:15

Authors (legacy)

Bence Ágg1, Zoltán V. Varga1, Anikó Görbe1, Péter Ferdinandy1
1 Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest