PhD Scientific Days 2022

Translational Medicine II. (Poster discussion will take place in the Aula during the Coffee Break)

The decompensation of systolic function in pressure overload-induced left ventricular myocardial hypertrophy is associated with unique microRNA expression profile

Előadó neve

Dr. Ruppert, Mihály

Előadó munkahelye

Heart and Vascular Center, Semmelweis University, Városmajor St. 68, Budapest, H-1122, Hungary

Előadó telefonszáma

+36206701233

Előadó e-mail címe

ruppertmis@gmail.com

Az előadás címe

The decompensation of systolic function in pressure overload-induced left ventricular myocardial hypertrophy is associated with unique microRNA expression profile

Szerző(k) neve és munkahelye

Mihály Ruppert1, Sevil Korkmaz-Icöz2, Bence Ágg3, Alex Ali Sayour1, Attila Oláh1, Dávid Nagy1, Tímea Bálint1, Péter Ferdinandy3, Béla Merkely1, Gábor Szabó2,4, Tamás Radovits1

1Heart and Vascular Centre, Semmelweis University, Budapest, Hungary
2Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany
3Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest
4Department of Cardiac Surgery, University Hospital Halle (Saale), Halle, Germany.

Bemutatás módja

Poszter

Szekció

Translational Medicine II. (Poster discussion will take place in the Aula during the Coffee Break)

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: microRNA (miRNA) expression is dysregulated in pressure overload (PO)-induced left ventricular (LV) myocardial hypertrophy (LVH). Nevertheless, whether the altered miRNA expression might contribute to the decompensation of LV systolic function is debated.
Aims: Hence, we aimed to characterize miRNA expression in PO-induced LVH with and without systolic heart failure (HF).
Methods: Aortic banding (AB) was performed in male rats to evoke PO. Sham-operated animals served as controls. Functional and morphological alterations were assessed by echocardiography and histology. At the end of the experimental period, rats in the AB group were subcategorized based on ejection fraction [EF] into ABLVH (EF>40%) and ABHF groups (EF<40%). Global miRNA expression profiling was performed using next generation sequencing. Bioinformatics analysis was carried out to predict miRNA-target interactions. Expression of selected target genes was measured by qRT-PCR.
Results: Increased heart weight-to-tibial length, LV mass and fibrosis confirmed the development of pathological LVH in both the ABLVH and ABHF groups. Nevertheless, increased lung weight-to-tibial length, chamber dilatation and severely reduced EF was noted only in the ABHF and not in the ABLVH, when compared to the sham group. 50 miRNA showed different expression in the ABHF compared to the ABLVH group. Based on the altered gene expression profile, in silico bioinformatics analysis predicted several target genes. Among them, reduced mRNA expression level of Fmr1 (FMRP translational regulator 1), Zfpm2 (zinc finger protein, multitype 2), Wasl (WASP like actin nucleation promoting factor), Ets1 (ETS proto-oncogene 1) and Atg16l1 (Autophagy Related 16 Like 1) was confirmed in ABHF compared to ABLVH.
Conclusions: Decompensation of systolic function in PO-induced LVH is associated with unique miRNA profile leading to specific regulation of gene expression.
Funding: New National Excellence Program of the Ministry of Human Capacities (ÚNKP-21-4-II-SE-5 to M.R.)

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Tamás Radovits MD, PhD

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1039

Start

13:40

End

13:45

Authors (legacy)

Mihály Ruppert1, Sevil Korkmaz-Icöz2, Bence Ágg3, Alex Ali Sayour1, Attila Oláh1, Dávid Nagy1, Tímea Bálint1, Péter Ferdinandy3, Béla Merkely1, Gábor Szabó2,4, Tamás Radovits1

1Heart and Vascular Centre, Semmelweis University, Budapest, Hungary
2Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany
3Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest
4Department of Cardiac Surgery, University Hospital Halle (Saale), Halle, Germany.