PhD Scientific Days 2022

Neurosciences I. (Poster discussion will take place in the Aula during the Coffee Break)

RetaiN: Neuroimmunology and stress resistance in human ageing and neurodegeneration

Előadó neve

Dr. Danics, Lea

Előadó munkahelye

Semmelweis University Institute of Translational Medicine

Előadó telefonszáma

+36206701162

Előadó e-mail címe

danics.lea@med.semmelweis-univ.hu

Az előadás címe

RetaiN: Neuroimmunology and stress resistance in human ageing and neurodegeneration

Szerző(k) neve és munkahelye

Anna Abbas /Semmelweis University
Balázs Kis / Semmelweis University
Dorina Jamniczky /Semmelweis University
Pálma Anna Zsolnai / Semmelweis University
Zoltán Veréb / University of Szeged
Thomas B. Stoker / University of Cambridge
Kate Harris / University of Cambridge
Shaline V. Fazal / University of Cambridge
Romina Vuono / University of Cambridge
Lajos Kemény / University of Szeged
Janelle Drouin-Ouellet / University of Montreal
Roger A. Baker / University of Cambridge
Karolina Pircs / Semmelweis University, Lund University

Bemutatás módja

Poszter

Szekció

Neurosciences I. (Poster discussion will take place in the Aula during the Coffee Break)

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

In our aging society there is an urgent need to understand aging, since it affects everyone and it has a great impact on our life and health. Changes of the immune functions and stress-protection pathways are detected in most of the diseases including aging-related disorders, like neurodegeneration. The role and importance of the altered protective mechanisms in these disorders and in general in human aging are still not explored and understood completely. We investigate the immunological and stress-protection-related mechanisms of the neurons in differently aged healthy individuals, using the induced neuron (iN) model. It is a novel tool to investigate human aging by direct reprogramming of fibroblasts into neurons. Uniquely, iNs retain the phenotype, epigenetics and aging signatures of the donor.
Our aim is to gain new insight into the physiology of human aging focusing on neuroimmunological and stress-protection-related mechanisms. The fibroblast cells used in our model are isolated from healthy individuals by skin biopsy. Our cohort consists of 6 young (1-34 years old), 8 middle-aged (41-61 years old) and 10 old (66-85 years old) donors. The isolated fibroblasts have been cultured under standard conditions and transformed into iNs using an “all-in-one” lentiviral vector for transduction. The purity and efficiency of the direct reprogramming have been identified using high-content automated microscopy analysis of MAP2- and TAU-immunolabelled iNs. Further characterization of the differently aged iNs will be done using high content screening, immunocytochemistry, western blot, ELISA, proteomic, transcriptomic and electrophysiological analysis.
With the help of this project we can get closer to understand the role of immune- and stress-protection mechanisms in aging, through which we can identify novel targets to prevent age-related diseases and achieve healthy aging.
Founding: ÚNKP-21, HCEMM, ICGEB, TKP-NVA20

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Karolina Pircs

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2733

Start

13:10

End

13:15

Authors (legacy)

Anna Abbas /Semmelweis University
Balázs Kis / Semmelweis University
Dorina Jamniczky /Semmelweis University
Pálma Anna Zsolnai / Semmelweis University
Zoltán Veréb / University of Szeged
Thomas B. Stoker / University of Cambridge
Kate Harris / University of Cambridge
Shaline V. Fazal / University of Cambridge
Romina Vuono / University of Cambridge
Lajos Kemény / University of Szeged
Janelle Drouin-Ouellet / University of Montreal
Roger A. Baker / University of Cambridge
Karolina Pircs / Semmelweis University, Lund University