PhD Scientific Days 2022

Neurosciences II.

The role of different neuron types in the median raphe region in depressive-like behaviour and concomitant changes in body temperature regulation

Előadó neve

Fazekas, Csilla Lea, MSc

Előadó munkahelye

Institute of Experimental Medicine

Előadó telefonszáma

+36305809327

Előadó e-mail címe

ghalla195@gmail.com

Az előadás címe

The role of different neuron types in the median raphe region in depressive-like behaviour and concomitant changes in body temperature regulation

Szerző(k) neve és munkahelye

Csilla Lea Fazekas1,2, Manon Bellardie1,3, Bibiána Török1,2, Adrienn Szabó 1,2, Eszter Sipos1, Elodie Chaillou3, Dóra Zelena1,4
1 Institute of Experimental Medicine, Budapest, Hungary
2 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary
3 INRAE Centre Val de Loire, CNRS, IFCE, Université de Tours, UMR 85 Physiologie de la Reproduction et des Comportements, France
4 Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary

Bemutatás módja

Szóbeli

Szekció

Neurosciences II.

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Introduction: The midbrain median raphe region (MRR) has been linked to numerous different behaviours and stress adaptation, but its effect on vegetative functions is debated. Additionally, it is unknown if there is connection between them. The MRR is mostly known for its serotonergic (SERT+) neurons, although they constitute a minor population of the nucleus. Interestingly, there is also a subpopulation of corticotrophin releasing hormone positive (CRH+) cells, which molecule is associated to stress.
Aims: Our aim was to investigate the role of MRR, especially its SERT+ and CRH+ neurons in depressive-like behaviour in parallel with changes in core body temperature (BT) as a vegetative function
Methods: Using pharmacogenetics control, excitatory and inhibitory designer receptors (DREADDs) were expressed in the mouse MRR. A biotelemetry system was implanted into the abdominal cavity to monitor changes in BT. Following injection of clozapine-N-oxide (CNO), the ligand for DREADDs, behavioural tests were performed. Depressive-like behaviour and cold stress reactivity was measured by the forced swim test (FST) at 23°C water temperature. The same protocol was repeated in SERT-Cre and CRH-Cre mice, expressing the DREADD only in the SERT+ or CRH+ cells of the MRR, respectively, but only with control and excitatory groups.
Results: The excitation of the MRR during FST increased floating, while marginally decreased struggling. The drop in BT was smaller after stimulating all neurones of the MRR, both during and after the test. In the SERT-Cre mice excitation of MRR SERT+ neurons marginally increased floating and significantly decreased struggling. Furthermore, the previously seen decrease in BT to FST after whole MRR stimulation was reproduced in the SERT-Cre excitatory group. The excitation of CRH+ cells did not affect the behaviour or the body temperature.
Conclusions: We showed that the MRR effectively regulates depressive-like behaviour as well as BT, in which SERT+, but not CRH+ neurons are involved. This finding may have clinical relevance in human depressive disorders.
Funding: Supported by the ÚNKP-21-3 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund; ERC-2011-ADG-294313; OTKA (grant numbers K120311, K131406); bilateral Balaton Program (TÉT_15-1-2016-003).

University and Doctoral School

Semmelweis University, János Szentágothai Doctoral School of Neurosciences

Supervisor

Dr. Dóra Zelena

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6947

Start

16:45

End

17:00

Authors (legacy)

Csilla Lea Fazekas1,2, Manon Bellardie1,3, Bibiána Török1,2, Adrienn Szabó 1,2, Eszter Sipos1, Elodie Chaillou3, Dóra Zelena1,4
1 Institute of Experimental Medicine, Budapest, Hungary
2 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary
3 INRAE Centre Val de Loire, CNRS, IFCE, Université de Tours, UMR 85 Physiologie de la Reproduction et des Comportements, France
4 Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary