PhD Scientific Days 2023

Molecular Sciences - Posters B

Neutrophil Effector Functions are Significantly Enhanced in the Presence of Bicarbonate

Előadó neve

Czárán, Domonkos, MSc

Neptun code

GE6M9K

Előadó munkahelye

Semmelweis University, Department of Physiology

Előadó telefonszáma

+36302643505

Előadó e-mail címe

czaran.domonkos@med.semmelweis-univ.hu

Az előadás címe

Neutrophil Effector Functions are Significantly Enhanced in the Presence of Bicarbonate

Szerző(k) neve és munkahelye

Domonkos Czárán1, Wuttapon Sadaeng2, Pongsiri Jaikumpun2, Zsófia Danka2, Roland Csépányi-Kömi1, Ákos Zsembery2
1 Semmelweis University, Department of Physiology, Budapest, Hungary
2 Semmelweis University, Department of Oral Biology, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Molecular Sciences - Posters B

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

Background: Recent studies have suggested, that bicarbonate has far more diverse effects than a simple buffer system. It has been described that it inhibits the growth and biofilm formation of different pathogen bacteria, increases the activity of antimicrobial peptides, and enhances bacterial susceptibility to certain antibiotics. Although HCO3- has been shown to regulate neutrophil extracellular trap (NET) formation, there is no data about its effects on other important neutrophil functions. Therefore, in our current study, we aim to investigate the impact of HCO3- on these functions using human polymorphonuclear leukocytes (PMN).
Methods: PMNs were isolated from the blood of healthy volunteers. The phagocytosis of serum opsonized S. Aureus was detected with flow cytometry. Superoxide burst of neutrophils was measured with lucigenin luminescence assay upon opsonized zymosan stimulus. The S. aureus killing capacity of PMN was assessed using the bacterium survival assay. The migration capacity of neutrophils was measured using transwell assay and IL-8 production was determined with the use of an ELISA kit. In each set of experiments, we used the following conditions: plain Hank’s Balanced Salt Solution (HBSS), or HBSS supplemented with 25 or 50 mM NaHCO3 in a 5% CO2 environment, and as adequate controls, plain HBSS or HBSS supplemented with 25 or 50 mM NaCl in an ambient air environment.
Results: Both 25 and 50 mM NaHCO3 significantly increased the phagocytosis capacity of neutrophils compared to osmotic controls, and this difference proved to be pH independent. Intracellular superoxide production and bacterium-killing capacity of PMN were also elevated by HCO3-. We found no effect of bicarbonate on the transmigrating capability and IL-8 production of neutrophils.
Conclusions: Apart from the previously described antibacterial properties of bicarbonate, our data suggest that HCO3- stimulates important effector functions of PMNs. We believe that these beneficial effects of HCO3- could complement each other making bicarbonate a very effective therapeutic agent in infectious diseases where clearance of bacteria by neutrophils is especially important (e.g., in cystic fibrosis).
Funding: EFOP-3.6.3-VEKOP-16-2017-00009, NKFIH FK_18/128376, TKP2021-EGA-24, Semmelweis 250+ Excellence PhD Scholarship

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Dr. Roland Csépányi-Kömi

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4698

Start

11:18

End

11:23

Authors (legacy)

Domonkos Czárán1, Wuttapon Sadaeng2, Pongsiri Jaikumpun2, Zsófia Danka2, Roland Csépányi-Kömi1, Ákos Zsembery2
1 Semmelweis University, Department of Physiology, Budapest, Hungary
2 Semmelweis University, Department of Oral Biology, Budapest, Hungary