PhD Scientific Days 2023

Translational Medicine I.

Non-steroid anti-inflammatory treatment enhances the efficacy of modulated electro hyperthermia on triple negative breast cancer and melanoma cancer models in vivo.

Előadó neve

Giunashvili, Nino

Neptun code

WYHKTU

Előadó munkahelye

Basic and Translational Medicine

Előadó telefonszáma

06705523059

Előadó e-mail címe

nino.giunashvili@phd.semmelweis.hu

Az előadás címe

Non-steroid anti-inflammatory treatment enhances the efficacy of modulated electro hyperthermia on triple negative breast cancer and melanoma cancer models in vivo.

Szerző(k) neve és munkahelye

Nino Giunashvili,1. PhD student, Institute of Basic and Translational Medicine.
Jeremiah Thomas, 2 Institute of Basic and Translational Medicine.
Pedro Viana, 2 Institute of Basic and Translational Medicine.
Kenan Aloss, 2 Institute of Basic and Translational Medicine.
Mahak Zahra, 2 Institute of Basic and Translational Medicine.
Lea Danics, 2 Institute of Basic and Translational Medicine.
Zoltan Koos, 2 Institute of Basic and Translational Medicine.
Daniel Bocsi, 2 Institute of Basic and Translational Medicine.
Eniko Major, 2 Institute of Basic and Translational Medicine.
Zoltán Benyó,2 Institute of Basic and Translational Medicine.
Csaba András Schvarcz,2 Institute of Basic and Translational Medicine.
Péter Hamar, supervisor, Institute of Basic and Translational Medicine.

Bemutatás módja

Szóbeli

Szekció

Translational Medicine I.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Modulated electro-hyperthermia (mEHT) is an advanced option in the hyperthermia field, applying a 13.56 MHz radiofrequency electromagnetic current to induce tumor-specific damage. This study investigates the mEHT-induced molecular effect and the potential of combination non-steroid anti-inflammatory drugs (NSAIDs) to enhance its anti-tumor effects in 4T1 triple-negative breast cancer (TNBC) and B16F10 melanoma mouse models.
Materials and methods: 4T1 TNBC and B16F10 melanoma cell lines were injected into Balb/C and C57BL/6 mice, respectively. They have been treated according to the protocol with only mEHT or mEHT combined with non-selective COX-inhibitors (Aspirin) or selective COX2 inhibitors (SC236). Tumor volume was monitored by ultrasound and a digital caliper. At the end of the experiments, mice were euthanized and tumors excised for molecular studies.
Results and discussion: Here we report that mEHT monotherapy stimulates local IL1-beta and IL6, and consequently cyclooxygenase 2 (COX 2) production. These effects could be considered as part of a self-defensive, wound-healing reaction of the tumor to protect itself from the mEHT-induced stress. In the present study, we combined mEHT with non-steroid anti-inflammatory drugs (NSAIDs), the non-selective (Aspirin), or the selective COX2 inhibitor (SC236) in vivo. All of these therapies have already demonstrated antitumor effects in various cancer models as monotherapies. Here we demonstrate that NSAID treatment synergistically increased the effect of mEHT in 4T1 TNBC. Tumor weight and tumor volume (measured by ultrasound and a digital caliper) were lowest, and the tumor destruction ratio (TDR) was the highest in the combination treated (NSAID + mEHT) groups. Tumor damage was accompanied by a significant increase in cleaved caspase-3 (cC3), suggesting an important role for apoptosis. Similarly, in the B16F10 melanoma model, lungs nodules were significantly less in mice treated with mEHT + Aspirin.
Conclusion: NSAIDs effectively enhance the mEHT anti-tumor effect in TNBC and melanoma cancer models; they increase tumor destruction, where apoptosis may play a role. Disecting the exact molecular mechanisms further is under our current investigation.

First author: Nino Giunashvili
nino.giunashvili@phd.semmelweis.hu
Supervisor: Peter Hamar
Funding: SE250+ scholarship EFOP-3.6.3-VEKOP-16-2017-00009

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Peter Hamar

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6857

Start

09:00

End

09:15

Authors (legacy)

Nino Giunashvili,1. PhD student, Institute of Basic and Translational Medicine.
Jeremiah Thomas, 2 Institute of Basic and Translational Medicine.
Pedro Viana, 2 Institute of Basic and Translational Medicine.
Kenan Aloss, 2 Institute of Basic and Translational Medicine.
Mahak Zahra, 2 Institute of Basic and Translational Medicine.
Lea Danics, 2 Institute of Basic and Translational Medicine.
Zoltan Koos, 2 Institute of Basic and Translational Medicine.
Daniel Bocsi, 2 Institute of Basic and Translational Medicine.
Eniko Major, 2 Institute of Basic and Translational Medicine.
Zoltán Benyó,2 Institute of Basic and Translational Medicine.
Csaba András Schvarcz,2 Institute of Basic and Translational Medicine.
Péter Hamar, supervisor, Institute of Basic and Translational Medicine.