PhD Scientific Days 2023

Molecular Sciences - Posters B

The investigation of a new BRET system to study protein interactions

Előadó neve

Dr. Orsolya Tünde, Kovács

Neptun code

GTH7EJ

Előadó munkahelye

Department of Physiology

Előadó telefonszáma

+36309624745

Előadó e-mail címe

kovacsorsolyatunde@gmail.com

Az előadás címe

The investigation of a new BRET system to study protein interactions

Szerző(k) neve és munkahelye

Orsolya Tünde Kovács1, Eszter Soltész-Katona2, Ádám Misák1, Szilvia Barsi1, András Tóth1,2,3, Dániel Tóth1, Péter Várnai1, László Hunyady1,2, Gábor Turu1,2
1Department of Physiology, Semmelweis University, Budapest,
2Institute of Enzymology, Eötvös Loránd Research Network, Research Centre for Natural Sciences, Budapest,
3Department of Internal Medicine and Haematology, Semmelweis University, Budapest

Bemutatás módja

Poszter

Szekció

Molecular Sciences - Posters B

Language of the presentation

Hungarian

Preferred session

Molecular Sciences

Összefoglaló szövege

Titration BRET measurements are commonly used to study dynamic interactions. The disadvantage of this system is that it is cumbersome and time-consuming. Our aim was to develop a system in which static interactions can be studied under dynamic conditions in a simple way.
We investigated the interaction of HTSF1 protein and β-arrestin protein. β-arrestin proteins terminate the G-protein signaling after activation of GPCRs (G-protein coupled receptors) and also orchestrate a new wave of signal transduction steps. They regulate a broad range of cellular processes e.g. migration, proliferation, differentiation, and apoptosis.
A rapamycin system was used in this new method, which binds FRB and FKBP molecular tags upon stimulation. HEKT cells were transfected with; L10-FRB-mVenus, FKBP-Barr2 or FKBP-Barr2-384del (pre-activated form), and HTSF1-RLuc8 or HTSF1mut-RLuc8 (β-arrestin binding S-T pattern is mutated). In our experiments, we also cotransfected various GRKs (G protein-coupled receptor kinases) to promote protein phosphorylation.
Our results show that the static interaction of HTSF1 with β-arrestin2 can be detected with good sensitivity in our BRET system and this system can be used to rapidly investigate interactions that are otherwise difficult to manipulate dynamically.
Supported by the ÚNKP-22-4-I New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Dr. Gábor Turu

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4124

Start

11:24

End

11:29

Authors (legacy)

Orsolya Tünde Kovács1, Eszter Soltész-Katona2, Ádám Misák1, Szilvia Barsi1, András Tóth1,2,3, Dániel Tóth1, Péter Várnai1, László Hunyady1,2, Gábor Turu1,2
1Department of Physiology, Semmelweis University, Budapest,
2Institute of Enzymology, Eötvös Loránd Research Network, Research Centre for Natural Sciences, Budapest,
3Department of Internal Medicine and Haematology, Semmelweis University, Budapest