PhD Scientific Days 2023
Budapest, 22-23 June 2023
Pharmaceutical Sciences - Posters E
Development and validation of an LC-MS/MS method for the quantification of 2,3-epoxypropyl isopropyl ether in rat plasma
Előadó neve
Aliz, Széles
Neptun code
D1K9I2
Előadó munkahelye
Doctoral School of Pharmaceutical Sciences, Semmelweis University; Research Centre for Natural Sciences; Toxi-Coop Ltd.
Előadó telefonszáma
+36204942641
Előadó e-mail címe
szeles.aliz.1997@gmail.com
Az előadás címe
Development and validation of an LC-MS/MS method for the quantification of 2,3-epoxypropyl isopropyl ether in rat plasma
Szerző(k) neve és munkahelye
Katalin Monostory
Research Centre for Natural Sciences, Magyar tudósok körútja 2, H-1117 Budapest, Hungary
Tibor Renkecz
Toxi-Coop Zrt., Magyar jakobinusok tere 4., H-1122 Budapest, Hungary
Bemutatás módja
Poszter
Szekció
Pharmaceutical Sciences - Posters E
Language of the presentation
English
Preferred session
Pharmaceutical Sciences
Összefoglaló szövege
2,3-epoxypropyl isopropyl ether is a frequently used material during epoxy resin manufacturing process. There is still little known about the attainable systemic concentration of such chemicals. Present work can provide valuable information to assist exposure assessment/biomonitoring in occupational safety studies.
To serve the bioanalytical need of genotoxicity study, a sensitive and selective LC–MS/MS method has been developed and fully validated for the quantification of 2,3-epoxypropyl isopropyl ether in rat plasma.
The LC-MS/MS method worked with gradient elution [mobile phase A (20mM ammonium acetate in ultrapure water) B (acetonitrile)] on a YMC-Triart C18 column (3μm, 2.1x75 mm) with YMC-Triart C18 (3μm, 2.1x10 mm) guard column at 0.4 mL/min flow rate. The gradient program was as follows: initial conditions were 10% B; held at 10% B for 0.5 min, raised to 90% B over the next 3 minutes, held at 90% B for 0.5 min, decreased to 10% B over the next 0.5 min and held at 10% B for 0.5 min for re-equilibration of the system prior to the next injection. The column temperature was 30 °C and the injection volume was 10 μL. The autosampler temperature was 10°C. 2-propanol was used as washing solvent to prevent carry-over. As internal standard neopentyl glycol diglycidyl ether was used since no stable isotope labelled analogue of the analyte was available. The Shimadzu LCMS-8060 triple quadrupole tandem mass spectrometer was operated in Multiple Reaction Monitoring (MRM) mode and the ammonium adduct form of the precursor ion was selected for fragmentation. MS acquisition was carried out in the ESI positive ionization mode.
The LLOQ for the analyte was 5 µg/mL over a 5–5000 µg/mL range. The plasma matrix did not cause any signal suppression. The mean recovery was 88.4 % and 85.4 % at high (4000 µg/mL) and low (15 µg/mL) level of concentration. The plasma samples are considered stable at 75C ± 10C for at least 38 days and can be subjected to 3 freeze-thaw cycles and for at least 4 hours on benchtop at room temperature.
Overall, a selective method was developed with a wide concentration range. In the future, this method will be applied in a pilot study, where a single dose (1000 mg/kg) of 2,3-epoxypropyl isopropyl ether will be administered orally to rats and toxicokinetic plasma sampling will be performed at 6 time-points.
The present work was supported by Doctoral School of Pharmaceutical Sciences (Semmelweis University), Research Centre for Natural Sciences and Toxi-Coop Limited Company.
University and Doctoral School
Semmelweis University, Doctoral School of Pharmaceutical Sciences
Supervisor
Katalin Monostory
Publication of my abstract
I do not give consent to the publication of my abstract on the website of the congress.
Kind
Szabad
Status
elfogadva
Accepted presentation method
poszter
Előadás fájl jóváhagyás
nem rendelkezett róla
Előadó
7412
Start
12:00
End
12:05
Authors (legacy)
Katalin Monostory
Research Centre for Natural Sciences, Magyar tudósok körútja 2, H-1117 Budapest, Hungary
Tibor Renkecz
Toxi-Coop Zrt., Magyar jakobinusok tere 4., H-1122 Budapest, Hungary