PhD Scientific Days 2023

Pathology - Posters C

Effects of Blunting Annexin A1 Expression in Small Cell Lung Cancer Cell Lines

Előadó neve

Dr. Paál, Ágnes

Neptun code

E8NM06

Előadó munkahelye

MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University

Előadó telefonszáma

+362052094926

Előadó e-mail címe

paal.agnes@phd.semmelweis.hu

Az előadás címe

Effects of Blunting Annexin A1 Expression in Small Cell Lung Cancer Cell Lines

Szerző(k) neve és munkahelye

Ágnes Paál1, Ákos Takács1, Zoltán V. Varga1, István Peták1, Anikó Görbe1, Péter Ferdinandy1, Dávid Dóra2
1MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University
2Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University

Bemutatás módja

Poszter

Szekció

Pathology - Posters C

Language of the presentation

English

Preferred session

Pathology and Oncology

Összefoglaló szövege

The first advancement in small cell lung cancer (SCLC) therapy since the 1990s was the recent addition of immune checkpoint inhibitors to chemotherapy as first-line treatment of extensive-stage disease. However, response and resistance to Immunotherapy are not characterized in SCLC. Previous studies identified novel molecular subtypes of SCLC with distinct immune infiltration patterns. Still, tumors with strong immune infiltration are often resistant to Immunotherapy. A possible mechanism behind this phenomenon is the overexpression of immunosuppressive molecules, such as annexin A1 (ANXA1). We hypothesized that silencing ANXA1 expression in cell culture reduces growth rate, which would highlight the role of ANXA1 in SCLC therapy outcome. We aimed to investigate the effect of silencing ANXA1 expression in an SCLC cell line.
An SCLC cell line with high ANXA1 expression (SW1271) was transfected with control or ANXA1 silencing lentiviral short hairpin RNA (shRNA) vector, the selection agent was 0,1% puromycin in culture medium. ANXA1 silencing was tested with western blot. Growth rate of transfected and native cells was compared with trypan blue exclusion assay. Statistical analysis was done with Kruskal-Wallis test followed by Dunn’s test for the western blots, and with mixed-effects analysis followed by Tukey’s test for the trypan blue exclusion assays. The n number was 6 in each group in the experiments.
Annexin A1 expression was stably silenced with lentiviral shRNA vector, shown by western blots. Growth rate of ANXA1 silenced cells was lower than the control cells, and the difference increased with time of incubation as seen in 120-hour-long trypan blue exclusion assays.
Silencing annexin A1 expression has therapeutic relevance in small cell lung cancer. Reduction of growth rate by annexin A1 silencing is a feature that can help understand Immunotherapy resistance of this aggressive malignancy.
The work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement no. 739593 and by a Momentum Research Grant from the Hungarian Academy of Sciences (LP- 2021-14), by the European Union's Horizon 2020 research and innovation programme under grant agreement No 739593, by grants from the National Research, Development and Innovation Office (NKFIH) of Hungary (K139237) and by the „SE250+ Kiválósági Ösztöndíj Pályázat.”

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Dr. Anikó Görbe

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7414

Start

11:12

End

11:17

Authors (legacy)

Ágnes Paál1, Ákos Takács1, Zoltán V. Varga1, István Peták1, Anikó Görbe1, Péter Ferdinandy1, Dávid Dóra2
1MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University
2Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University