PhD Scientific Days 2023

Neurosciences - Posters H

Age-related Changes of Microglia in Wistar Rats

Előadó neve

Khochanskiy, Dmitry, PhD

Neptun code

BTKD-NY-NPAN-1

Előadó munkahelye

Semmelweis University

Előadó telefonszáma

+972 534367414

Előadó e-mail címe

demetros87@gmail.com

Az előadás címe

Age-related Changes of Microglia in Wistar Rats

Szerző(k) neve és munkahelye

Sentyabreva Alexandra V. 1,2; Khochanskiy Dmitry N. 3; Miroshnichenko Ekaterina A. 1,2; Tsvetkov Ivan S.1; Kosyreva Anna M 1,2.
1 Avtsyn Research Institute of human morphology of "Petrovsky National Research Centre of Surgery", Moscow
2 Research Institute of Molecular and Cellular Medicine, Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN)
3 Heart and Vascular Centre, Semmelweis University, Faculty Of Medicine, Budapest

Bemutatás módja

Poszter

Szekció

Neurosciences - Posters H

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Alzheimer’s disease (AD) is one of the most prevalent types of neurodegenerative diseases leading to dementia. There is still no consensus concerning the initial mechanisms of AD pathogenesis. The impact of inflammaging, or chronic age-related low-grade systemic inflammation, is one of the most perspective hypotheses so far. According to recent data microglia, which are resident immune cells in the CNS, might play the key role of AD development as well, and these cells also undergo the processes of cellular senescence.
The purpose of this work was to study morphofunctional changes of microglia in adult and old Wistar rats to reveal age-related alterations.
The work was performed on adult (n=10, age 3 months) and old (n=10, age 24 months) male Wistar rats. For ICH-P study, frontal histological sections of brains were stained with rabbit antibodies Iba1 and secondary HRP Donkey-anti-Rabbit antibodies. The expression of Il-6, Tnf-α, Il-10, Tgf-β, iNos, and Mmp9 mRNA was assayed by real-time qPCR in tissue fragments of the prefrontal cortex. The results were analyzed by Statistica 8.0 software (StatSoft, Inc.) using Mann-Whitney U-Test.
In a morphological study, adult rats’ microglia had a regular size and thin ramified processes, which are features of resting functional state. At the same time, old rats’ microglia had an increased size and spheroidal swelling, hypertrophic, beaded, and tortuous processes. The result of qPCR showed that Il-6 and Tnf-α expression levels were higher in old rats than in adult rats by 1.5-fold and 3-fold accordingly. Also iNos, which is a marker of M1 activated microglia, was 5-fold in old rats in comparison with adult rats. Old rats group showed no Il-10 expression at all, whereas the expression level of Tgf-β was 7.25-fold higher in adult rats. At the same time Mmp9, which is a marker of M2 activated microglia, was also 3-fold in old rats group.
Hence, obtained data demonstrate morphological changes of microglial cells, the decreasing of anti-inflammatory cytokines expression levels and the increasing of pro-inflammatory ones as well as microglia activation markers in old rats. This study confirms a great deal of differences between adult and old rodents’ physiological state. Since old rats demonstrate age-related changes similar to humans’, using aged animals for modeling of neurodegeneration is supposed to be more relevant.
Number of state registration of research, development, and technological work for civil purposes—122030200530-6

University and Doctoral School

Semmelweis University, Doctoral School of Health Sciences

Supervisor

Kosyreva Anna M

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1229

Start

11:06

End

11:11

Authors (legacy)

Sentyabreva Alexandra V. 1,2; Khochanskiy Dmitry N. 3; Miroshnichenko Ekaterina A. 1,2; Tsvetkov Ivan S.1; Kosyreva Anna M 1,2.
1 Avtsyn Research Institute of human morphology of "Petrovsky National Research Centre of Surgery", Moscow
2 Research Institute of Molecular and Cellular Medicine, Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN)
3 Heart and Vascular Centre, Semmelweis University, Faculty Of Medicine, Budapest