PhD Scientific Days 2023

Pharmaceutical Sciences II.

Developement of Corticosteroid-loaded Liposomes

Előadó neve

Dr. Budavári, Bálint Péter

Neptun code

OUC5ML

Előadó munkahelye

Department of Biophysics and Radiation Biology

Előadó telefonszáma

+36309562776

Előadó e-mail címe

balint.budavari@gmail.com

Az előadás címe

Developement of Corticosteroid-loaded Liposomes

Szerző(k) neve és munkahelye

Bálint Budavári, Department of Biophysics and Radiation Biology, Budapest
Angéla Jedlovkszky-Hajdú, Department of Biophysics and Radiation Biology, Budapest
Krisztina S. Nagy, Department of Biophysics and Radiation Biology, Budapest

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences II.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

Introduction: Liposomes are nanoscale drug delivery systems that offer many advantages regarding biodistribution, absorption and controlled drug release. Corticosteroids as lipophilic active agents integrate into the lipid bilayer. This novel approach can improve the efficacy of several anti-inflammatory therapies.
Aims: Our goals were to create long-term stable liposomes which can incorporate and release corticosteroids at inflamed body temperature. Considering the possible future application in asthma therapy, we also aimed at reaching high entrapment efficacy and slight drug leakage of the vesicles.
Methods: Two kinds of liposome samples were prepared from three different kinds of phospholipids by the widely used thin layer hydration method and subsequent extrusion to get small unilamellar vesicles. Prednisolone (Pred) and budesonide (Bud) were used as active agents. Stability tests were executed by dynamic light scattering while entrapment efficacy was determined by size-exclusion gel chromatography. The effect of liposomal drugs on cell viability was measured on the EBC-1 human lung carcinoma cell line. The internalization of the liposomes was studied by flow cytometry and confocal microscopy.
Results: Unilamellar vesicles with 100 nm in diameter were successfully prepared. Their hydrodynamic diameter has remained in the desired range through 6 months in case of the vast majority of the samples. It turned out that the type of corticosteroid has only a weak effect on the stability of the liposomes but their presence is more important. The entrapment efficiency was exceptionally high in both cases (above 90%) and the drug leakage was 35-40% for Pred and 6-8% for Bud in the first 30 min. Neither the free corticosteroids nor their liposomal form affected significantly the cell viability. Confocal microscopy images and flow cytometry results proved equally the internalization of the vesicles by the cells.
Conclusion: We have successfully created corticosteroid-loaded liposomes with long-term stability (6 months) and excellent entrapment efficiency due to the lipophilic character of the applied drugs. Furthermore, they are not toxic and can be internalized by the investigated pulmonary cells.
Funding: Semmelweis 250+ Excellence PhD Scholarship, EFOP-3.6.3-VEKOP-16-2017-00009, NKFI FK 124147, TKP2021-EGA-23

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Dr. Simonné Dr. Nagy Krisztina

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7441

Start

16:00

End

16:15

Authors (legacy)

Bálint Budavári, Department of Biophysics and Radiation Biology, Budapest
Angéla Jedlovkszky-Hajdú, Department of Biophysics and Radiation Biology, Budapest
Krisztina S. Nagy, Department of Biophysics and Radiation Biology, Budapest