PhD Scientific Days 2023

Pathology I.

Prognostic role of IL-1β, IL-18, and their antagonist proteins in pancreatic neoplasms

Előadó neve

Dr. Németh, Kristóf

Neptun code

G0G7P4

Előadó munkahelye

Department of Pathology, Forensics and Insurance Medicine- Semmelweis University

Előadó telefonszáma

06304026645

Előadó e-mail címe

nemkikko@gmail.com

Az előadás címe

Prognostic role of IL-1β, IL-18, and their antagonist proteins in pancreatic neoplasms

Szerző(k) neve és munkahelye

Dr. Németh Kristóf 1, Mezei Eszter 1

1 Department of Pathology, Forensics and Insurance Medicine- Semmelweis University

Bemutatás módja

Szóbeli

Szekció

Pathology I.

Language of the presentation

Hungarian

Preferred session

Pathology and Oncology

Összefoglaló szövege

Introduction: IPMN is the most common cystic neoplasm of the pancreas. The clinical relevance of the lesion is due to its increased incidence, early detectability by imaging technics, malignant transformation tendency, and better prognosis compared with conventional pancreatic ductal adenocarcinoma (PDAC). IL-1β and IL-18 production has been extensively investigated in PDAC, but its role in tumor progression in IPMN has not been investigated. Aims: The aim of the present research is to determine the effect of the effector molecules and their antagonist proteins in inflammasome activation on survival and the pathological properties of IPMN and PDAC. Methods: In our study, we examined histological specimens from patients diagnosed with IPMN (n=99) and PDAC (n=89) at the Department of Pathology, Forensics and Insurance Medicine between 2008 and 2021. Tissue microarrays (TMA) were prepared from the samples and labeled by immunohistochemistry using antibodies to NLRP3, Asc, caspase-1, IL-1β, IL-18, IL-1Ra, IL-18bp, and sections were digitized and evaluated using a semi-automated image analysis method. Patient data were collected from the institute's internal database (MedRec), the electronic health record system of Semmelweis University, and the database of the Central Statistical Office. Results: Asc, IL-1β, and IL-18 proteins showed higher expression in PDAC compared to IPMN (Asc: p<0.0001, IL-1β: p<0.0001, IL-18: p=0.0097 ). Compared to the other two subtypes at the intestinal metaplasia of IPMN lower IL-18 and higher IL-18bp expression are observed. (gastric vs. intestinal p=0.006, pancreaticobiliary vs. intestinal p=0.0018) Looking at all cases, significantly shorter survival was observed with high Asc, IL-1beta, and IL-18 expression. (Asc: p=0.02, IL-1beta: p=0.05, IL-18: p=0.00024) When only invasive cases were considered, high IL-18 and low IL-18bp expression were significantly associated with shorter survival (IL-18: p=0.003, IL-18bp:p=0.034). Conclusion and Funding: Higher inflammasome activity is observed in PDAC compared to IPMN. Lower IL-18 expression is likely to underlie the improved prognosis in intestinal-type IPMN. IL-18 and IL-18bp protein levels are important independent prognostic factors in pancreatic carcinomas.

University and Doctoral School

Semmelweis University, Doctoral School of Pathological Sciences

Supervisor

Dr. Budai András

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6906

Start

09:45

End

10:00

Authors (legacy)

Dr. Németh Kristóf 1, Mezei Eszter 1

1 Department of Pathology, Forensics and Insurance Medicine- Semmelweis University