PhD Scientific Days 2023

Molecular Sciences III.

Investigating the Heat Shock Protein 90 Interactome in C. elegans

Előadó neve

Saba, Khatatneh, MSc

Neptun code

EU8CCM

Előadó munkahelye

Semmelweis University

Előadó telefonszáma

+36704115915

Előadó e-mail címe

S.t.khatatneh@live.com

Az előadás címe

Investigating the Heat Shock Protein 90 Interactome in C. elegans

Szerző(k) neve és munkahelye

Saba Khatatneh, PhD student, Department of Molecular Biology/Semmelweis University, Budapest
Dr Milán Somogyvári, Assistant Professor, of Molecular Biology/Semmelweis University, Budapest
Dr Csaba Sőti, Associate professor, Department of Molecular Biology/Semmelweis University, Budapest

Bemutatás módja

Szóbeli

Szekció

Molecular Sciences III.

Language of the presentation

English

Preferred session

Molecular Sciences

Összefoglaló szövege

Our ongoing project aims to investigate the interactome of Heat Shock Protein 90 (Hsp90), a conserved molecular chaperone that plays critical roles in various cellular processes by keeping certain proteins, called clients, in a functional form. The interactome of Hsp90 is already well studied in mammals, whereas our knowledge of it in the widely used Caenorhabditis elegans model-system is lacking. Our study aims to elucidate this interactome using TurboID-based proximity labeling to uncover its functions and potential therapeutic relevance, and to understand the roles of Hsp90 in specific tissues, contributing to our understanding of Hsp90-related pathologies.
We utilize a promiscuous biotin ligase called TurboID fused to Hsp90, driven by an inducible Tet/Q Hybrid expressional system, to specifically biotinylate Hsp90 interactors and identify them. Tissue-specific analyses using specific promoters expressed in germ cells, muscles, intestines, and neurons will reveal tissue-specific subsets of Hsp90 clients, shedding light on tissue-specific functions. We will investigate potential connections between Hsp90 and other important proteins/pathways such as DAF-16/FOXO, SIR-2.1/Sirt1, and autophagy. We designed and created a stable C. elegans strain expressing HSP-90::TurboID fusion protein using the GeneGun method. We’ll isolate biotinylated proteins using streptavidin beads and perform mass spectrometry analysis to identify and characterize Hsp90 interactors. The data obtained will likely reveal a network of Hsp90-interacting proteins in different tissues and provide insights into Hsp90's roles in addition to tissue-specific functions. We plan to validate novel interactions using molecular and physiological methods to better understand the biological function of Hsp90 in various pathologies.

Funding for this work comes from OTKA PD 142838 research grant.

University and Doctoral School

Semmelweis University, Doctoral School of Molecular Medicine

Supervisor

Dr Milán Somogyvári and Dr Csaba Sőti

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7491

Start

13:00

End

13:15

Authors (legacy)

Saba Khatatneh, PhD student, Department of Molecular Biology/Semmelweis University, Budapest
Dr Milán Somogyvári, Assistant Professor, of Molecular Biology/Semmelweis University, Budapest
Dr Csaba Sőti, Associate professor, Department of Molecular Biology/Semmelweis University, Budapest