PhD Scientific Days 2023

Health Sciences II.

Physiological Significance of the Endocannabinoid System and its Role in Hypertension

Előadó neve

Shenker-Horváth, Kinga, MSc

Neptun code

MNTW4N

Előadó munkahelye

Hungarian University of Sports Science, Center for Sports Nutrition Science, Budapest; Department of Morphology and Physiology, Faculty of Health Sciences, Semmelweis University, Budapest,

Előadó telefonszáma

+36302430796

Előadó e-mail címe

shenkerhorvathkinga@gmail.com

Az előadás címe

Physiological Significance of the Endocannabinoid System and its Role in Hypertension

Szerző(k) neve és munkahelye

Kinga Shenker-Horváth1,2, Zsolt Vass2, Judit Kiss2, Margita Szilágyi-Utczás1, György Nádasy3, Péter Hamar4, Gábor Szénási4, László Dézsi4, László Hunyady3,5, Gabriella Dörnyei2, Mária Szekeres2,3
1 Hungarian University of Sports Science, Center for Sports Nutrition Science, Budapest
2 Semmelweis University, Faculty of Health Sciences, Department of Morphology and Physiology, Budapest
3 Semmelweis University, Faculty of Medicine, Department of Physiology, Budapest
4 Semmelweis University, Faculty of Medicine, Translational Medicine Institute, Budapest
5 Eötvös Loránd Research Network Natural Science Research Center, Institute of Enzymology, Budapest

Bemutatás módja

Szóbeli

Szekció

Health Sciences II.

Language of the presentation

Hungarian

Preferred session

Health Sciences

Összefoglaló szövege

Introduction: The endocannabinoid system (ECS) regulates cell proliferation, differentiation, and apoptosis. Endocannabinoids (eCBs) are produced primarily in the central nervous system and affect many functions of the body. eCBs are also synthesised in peripheral tissues (e.g. heart, blood cells, adipose tissue, muscles) and affect both energy balance and blood circulation. The role of ECS has also been demonstrated in a number of pathological processes (cardiovascular /CV/ diseases, obesity, diabetes, tumour formation). The main target of action is the cannabinoid CB1 receptor (CB1R), its density is altered in CV diseases, raising the therapeutic potential of ECS modulation. Angiotensin II (Ang II) plays a role in many forms of hypertension. We have previously shown that the vasoconstrictor effect of Ang II is attenuated by endocannabinoids through activation of CB1R.

Aims: We investigated the role of CB1 receptors in the development of Ang II-induced hypertension.

Method: Our experiments were performed in male C57BL/6N, CB1R gene-deficient (CB1-/-) and wild-type (WT, CB1+/+) mice. A continuous delivery of Ang II (800 ng/kg/min) was provided in mice for two weeks by subcutaneous osmotic minipump implantation. Animals were anaesthetized (Euthasol 50 mg/kg ip), their blood pressure (MAP) was measured invasively and aortic tissue samples from the animals were isolated and placed in 4% paraformaldehyde. The samples were subjected to orcein histochemical staining and elastin density of the vessel wall was determined.

Results: Two weeks of Ang II infusion in anaesthetized mice significantly increased MAP (W control: 74.2±6 mmHg, Ang II-treated: 117.1±12 mmHg, p<0.05). Using orcein immunohistochemistry, we showed that optical density (OD) in the aorta of Ang II-infused CB1+/+ mice was lower than in normotensive mice (p<0.05), indicating a reduction in the proportion of elastic fibres in Ang II-induced hypertension. However, the reduced OD in hypertension was enhanced in the absence of CB1 receptors (p<0.05).

Conclusion: Our results suggest that the impaired vascular function observed in Ang II-induced hypertension is mediated in part by CB1R receptors, which may open up new therapeutic strategy by selectively blocking CB1R activity.

Funding: OTKA K116954, K139231; ÚNKP22-3-II-SE-6

Keywords: endocannabinoid system, CB1 receptor, hypertension, angiotensin II, immunohistochemistry

University and Doctoral School

Semmelweis University, Doctoral School of Health Sciences

Supervisor

Mária Szekeres MD, PhD and Gabriella Dörnyei PhD

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

3880

Start

16:00

End

16:15

Authors (legacy)

Kinga Shenker-Horváth1,2, Zsolt Vass2, Judit Kiss2, Margita Szilágyi-Utczás1, György Nádasy3, Péter Hamar4, Gábor Szénási4, László Dézsi4, László Hunyady3,5, Gabriella Dörnyei2, Mária Szekeres2,3
1 Hungarian University of Sports Science, Center for Sports Nutrition Science, Budapest
2 Semmelweis University, Faculty of Health Sciences, Department of Morphology and Physiology, Budapest
3 Semmelweis University, Faculty of Medicine, Department of Physiology, Budapest
4 Semmelweis University, Faculty of Medicine, Translational Medicine Institute, Budapest
5 Eötvös Loránd Research Network Natural Science Research Center, Institute of Enzymology, Budapest