PhD Scientific Days 2023

Pharmaceutical Sciences - Posters F

Prussian Blue Nanoparticles as isotope-labeled and fluorescent contrast material

Előadó neve

Dr. Laszlo, Forgach

Neptun code

T9PTOD

Előadó munkahelye

SU Dpt. of Biophysics and Radiation Biology

Előadó telefonszáma

+36308921791

Előadó e-mail címe

laszlo.forgach@gmail.com

Az előadás címe

Prussian Blue Nanoparticles as isotope-labeled and fluorescent contrast material

Szerző(k) neve és munkahelye

László Forgách1, Nikolett Hegedűs1, Anikó Kubovje1, Zoltán Varga1,2, Noémi Kovács1,3, Ildikó Horváth1, Domokos Máthé1,3, Krisztián Szigeti1
1: Dept. Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
2: Research Center for Natural Sciences, ELKH, Budapest, Hungary
3: In Vivo Imaging ACF, Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary

Bemutatás módja

Poszter

Szekció

Pharmaceutical Sciences - Posters F

Language of the presentation

English

Preferred session

Pharmaceutical Sciences

Összefoglaló szövege

INTRODUCTION Prussian Blue Nanoparticles (PBNPs) have proven their capabilities as radiolabeled and fluorescently labelled nanoparticulate systems,. The mesoporous structure allows the doping of radioisotopes into the PBNP crystalline, while the net negative charge helps the conjugation of the positively charged IR820 onto the nanoparticle surface.
AIMS The study presents the synthesis and development of a Prussian Blue based biocompatible and chemically stable T1 magnetic resonance imaging (MRI) contrast agent with near infrared (NIR) optical contrast for preclinical application.
METHODS The physical properties of the Prussian blue nanoparticles (PBNPs) (iron (II); iron (III);octadecacyanide) were characterized with dynamic light scattering (DLS), zeta potential measurement, atomic force microscopy (AFM), and transmission electron microscopy (TEM). In vitro contrast enhancement properties of PBNPs were determined by MRI.
RESULTS In vivo T1-weighted contrast of the prepared PBNPs was investigated by MRI and optical imaging modality after intravenous administration to mice. PBNP-s doped with 201Tl isotope have also been synthetised and biodistribution in mice was determined by using SPECT and MRI. Activity concentrations (MBq/cm3) were calculated from the SPECT scans for each dedicated volume of interest (VOI) of liver, kidneys, salivary glands, heart, lungs, and brain. Isotope-labeled citrate-coated PBNP accumulation peaked at 2 hours after injection in the kidneys and the liver followed by a gradual decrease in activity in later time points.
CONCLUSION We synthetized, characterized, and radiolabeled a Prussian blue-based nanoparticle platform for contrast material applications. In vivo radiochemical stability and biodistribution open up the way for further diagnostic applications. The presence and detectability of PBNPs during initial biodistribution in the cardiovascular system indicates vascular contrast material applications, too.
FUNDING The research was financed by the Higher Education Institutional Excellence Programme of the Ministry for Innovation and Technology in Hungary, within the framework of the Therapeutic Development thematic programme of the Semmelweis University. The study was supported by EFOP-3.6.3-VEKOP-16-2017-00009 grant of the National Research, Development and Innovation Office, Hungary. The research leading to these results has received funding from the Semmelweis University (STIA-KFI-2020).

University and Doctoral School

Semmelweis University, Doctoral School of Pharmaceutical Sciences

Supervisor

Dr. Krisztian Szigeti

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

5927

Start

12:00

End

12:05

Authors (legacy)

László Forgách1, Nikolett Hegedűs1, Anikó Kubovje1, Zoltán Varga1,2, Noémi Kovács1,3, Ildikó Horváth1, Domokos Máthé1,3, Krisztián Szigeti1
1: Dept. Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
2: Research Center for Natural Sciences, ELKH, Budapest, Hungary
3: In Vivo Imaging ACF, Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary