PhD Scientific Days 2023

Pathology - Posters D

Development of tumor-stroma co-cultures – lessons from 3D cultures of stable normal cell lines

Előadó neve

Baranyi, Marcell, PhD

Neptun code

H9DADP

Előadó munkahelye

Semmelweis University, Department of Pathology, Forensic and Insurance Medicine

Előadó telefonszáma

06307337608

Előadó e-mail címe

baranyi2marci@gmail.com

Az előadás címe

Development of tumor-stroma co-cultures – lessons from 3D cultures of stable normal cell lines

Szerző(k) neve és munkahelye

Marcell Baranyi1, Fanni Bordás1, Eszter Molnár1, József Tímár1

1 Semmelweis University, Department of Pathology, Forensic and Insurance Medicine

Bemutatás módja

Poszter

Szekció

Pathology - Posters D

Language of the presentation

Hungarian

Preferred session

Pathology and Oncology

Összefoglaló szövege

Introduction
Development of successful anti-cancer modalities requires adequate and reliable models that can predict tumor responses. One of the most rapidly developing field of cancer research is co-culture systems and organoid technology.
Aims
Aim of this study was to establish complex 3D co-culture organoid-like systems using stable pancreatic tumor and normal cell lines and to compare them with xenograft tumors.
Methods
SW1990 pancreatic tumor, HUVEC-TERT endothelial, CCD986SK and LX2 fibroblast cell lines were used in this study. 2D and 3D cultures and in vivo SW1990 xenografts were treated with MRTX1133 KRAS G12D inhibitors. 3D organoids with or without normal cells were embedded into paraffin and were histologically analysed and compared to xenograft samples.
Result
SW1990 organoids showed high similarity to in vivo xenografts histological architecture and in response to MRTX1133 treatment. Incorporation and survival of normal cells, especially endothelial cells into organoids are challenging and requires specific ECM components.
Conclusion
Organoid co-culture model of SW1990 may serve as a useful tool to predict tumor response to KRAS targeting. Optimization of 3D culture conditions of stable normal cell lines may serve as a valuable complex in vitro tumor model for investigation of other anti-cancer modalities as well.
Funding
This study was supported by ÚNKP-22-4-II-SE-11 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund.

University and Doctoral School

Semmelweis University, Doctoral School of Pathological Sciences

Supervisor

József Tímár DSc

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2709

Start

11:42

End

11:47

Authors (legacy)

Marcell Baranyi1, Fanni Bordás1, Eszter Molnár1, József Tímár1

1 Semmelweis University, Department of Pathology, Forensic and Insurance Medicine