PhD Scientific Days 2023

Translational Medicine II.

Investigation of bladder overactivity in a novel mouse model

Előadó neve

Dr. Kinga, Borsodi

Neptun code

IWA1O0

Előadó munkahelye

Institute of Translational Medicine, Semmelweis University

Előadó telefonszáma

+36205740715

Előadó e-mail címe

kingaborsodi@gmail.com

Az előadás címe

Investigation of bladder overactivity in a novel mouse model

Szerző(k) neve és munkahelye

Kinga Borsodi1, Ádám Lénárt1, Helga Balla1, Péter József Molnár1,2, Zoltán Benyó1*
1 Institute of Translational Medicine, Semmelweis University, Budapest, Hungary
2 Department of Urology, Semmelweis University, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Translational Medicine II.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Overactive bladder syndrome (OAB) affects 17% of the European population, still its medical treatment has not yet been entirely resolved. The currently available animal models of OAB are often established by using suprapubic catheterization, in this way disrupting the bladder smooth muscle’s integrity.
Aims: Our aim was to set up a novel animal model mimicking the symptoms of OAB, which could contribute to finding potentially more specific pharmacological targets for treating bladder dysfunctions.
Methods: Experiments were performed on 90-120 days old, female, C57Bl/6 mice with applying transurethral catheterization under isoflurane anesthesia. For control (CTRL) experiments, the bladders were filled with 0.9% saline solution while measuring intravesical pressure changes. Following the control experiments, a group of mice was treated intravesically with 0.5% hydrogen-peroxide (H2O2) solution (30 min) and cystometric measurements were performed after 3h and 24h. Another group was treated with i.p. cyclophosphamide (CYP) solution (200mg/kg) and cystometry was performed 24h following treatment.
Results: We found that the amplitude of non-voiding contractions (NVC) elevated significantly following H2O2-(3h and 24h) and CYP-treatment. The frequency of NVCs reduced as a result of CYP-treatment but remained unaltered after H2O2-administration. Frequency of the voiding contractions (VC) also increased significantly following either treatment compared to CTRL, whereas the amplitude of VCs did not change. Furthermore, following H2O2- and CYP-treatment, the first VC and micturition appeared at significantly lower intravesical volumes compared to CTRL measurements.
Conclusion: We successfully set up a novel mouse model for OAB using transurethral catheterization allowing us to investigate bladder function with undisturbed integrity of the detrusor muscle layer. With either H2O2- or CYP-administration, the mice produced OAB-like symptoms, shown by the increase in the amplitude of NVCs, and the frequency of VCs. Furthermore, VCs and the first micturition appeared at lower volumes as a result of treatment, indicating bladder overactivity. In summary, these mouse models may aid the identification of novel, more specific therapeutic targets for treating overactive bladder.
Funding: NKFIH K-125174, K-135683 and K-139230 and EFOP-3.6.3-VEKOP-16-2017-00009 grants.

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Zoltán Benyó, MD, PhD, DSc

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4811

Start

12:15

End

12:30

Authors (legacy)

Kinga Borsodi1, Ádám Lénárt1, Helga Balla1, Péter József Molnár1,2, Zoltán Benyó1*
1 Institute of Translational Medicine, Semmelweis University, Budapest, Hungary
2 Department of Urology, Semmelweis University, Budapest, Hungary