PhD Scientific Days 2023

Neurosciences I.

Activation of VGLUT3 positive neurons in the median raphe region facilitate long-term memory formation in spatial learning task

Előadó neve

Fazekas, Csilla Lea

Neptun code

EMYGM9

Előadó munkahelye

University of Pécs, Medical School, Institute of Physiology, Laboratory of Behavioural and Stress Studies

Előadó telefonszáma

+36305809327

Előadó e-mail címe

ghalla195@gmail.com

Az előadás címe

Activation of VGLUT3 positive neurons in the median raphe region facilitate long-term memory formation in spatial learning task

Szerző(k) neve és munkahelye

Csilla Lea Fazekas1,2,3,4, Bibiána Török1,2, Adrienn Szabó1,2,3, Pedro Correia1,2,3,Tiago Chaves1,2,3, Stéphanie Daumas4 and Dóra Zelena1,2
1 Institute of Experimental Medicine, Budapest, Hungary;
2 Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary
3 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary
4 Sorbonne Université, Neuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS) INSERM, CNRS, Paris, France

Bemutatás módja

Szóbeli

Szekció

Neurosciences I.

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Introduction: The median raphe region (MRR) is classically considered a serotonergic nucleus, even though a significant subpopulation of neurons is actually positive for the vesicular glutamate transporter type 3 (VGLUT3). Moreover, it is known that these cells project to the hippocampus and influence its oscillations. Our aim was to characterise the behavioural effects of MRR-VGLUT3+ neuronal activity during spatial learning and memory formation.
Methods: We utilised chemogenetics (DREADDs) and optogenetics (ChR2) in VGLUT3-Cre mice in the hippocampus dependent Morris water maze paradigm. VGLUT3-Cre male and female mice were injected with Cre-dependent adenoassociated viral vectors containing either control or excitatory sequences. During spatial reference memory (SRM) phase the ligand clozapine-N-oxide was injected daily to the chemogenetic groups. For optogenetics, excitation was at 20 Hz for 10 sec while on the platform. Short-term memory was assessed 10 mins after last learning trial, while long-term memory was studied at 72 hours.
Results: Manipulations of MRR-VGLUT3+ neurons did not impact learning in SRM task. Chemogenetic manipulation resulted in no differences between the groups in short-term memory. However, 72 hours later, the excitatory group performed similarly well as before and found the place of the platform significantly faster than controls. Previous optogenetic excitation did not affect memory retrieval during the probes. These results were confirmed by proper quadrant preference during short-term memory test for all groups, which was maintained only in the chemogenetically stimulated group during long-term memory probe.
Conclusion: Our data showed a role of MRR-VGLUT3+ neurons in long-term memory formation for which long, extended excitation is required.
Funding: This project was supported by Campus France, SE 250+ Excellence Scholarship, bilateral Balaton Program (2021-1.2.4-TÉT-2021-00010) and National Research Development and Innovation Office of Hungary (grant numbers K141934, K138763).

University and Doctoral School

Semmelweis University, János Szentágothai Doctoral School of Neurosciences

Other university and doctoral school, not listed above

Sorbonne Université

Supervisor

Stéphanie Daumas, Dóra Zelena

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6947

Start

09:30

End

09:45

Authors (legacy)

Csilla Lea Fazekas1,2,3,4, Bibiána Török1,2, Adrienn Szabó1,2,3, Pedro Correia1,2,3,Tiago Chaves1,2,3, Stéphanie Daumas4 and Dóra Zelena1,2
1 Institute of Experimental Medicine, Budapest, Hungary;
2 Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary
3 János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary
4 Sorbonne Université, Neuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS) INSERM, CNRS, Paris, France