PhD Scientific Days 2023

Translational Medicine - Posters O

Effect of CB-1 receptor deficiency on morphological and functional changes in relaxation mechanisms of the female mouse aortic wall

Előadó neve

Dr. Banyai, Balint

Neptun code

OMZNHY

Előadó munkahelye

SE Institute of Physiology

Előadó telefonszáma

+36705783752

Előadó e-mail címe

banyai.balint@gmail.com

Az előadás címe

Effect of CB-1 receptor deficiency on morphological and functional changes in relaxation mechanisms of the female mouse aortic wall

Szerző(k) neve és munkahelye

Bálint Bányai1; Stella Tímea Kiss1,2; Gabriella Dörnyei2; Mária Szekeres2; Eszter Mária Horváth1
1- Semmelweis University Department of Physiology Budapest
2- Semmelweis University Morfological and physiological institute Budapest

Bemutatás módja

Poszter

Szekció

Translational Medicine - Posters O

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: The endocannabinoid system (ECS) is well known for its psychoactive effects, but it also act on the female reproductive system. Cannabinoids are involved in the regulation of the hypothalamo-pituitary-ovarian axis (HPO) and estrogen production. Estrogens have also been shown to significantly modulate cardiovascular function. In our previous experiments, we have shown increased estrogen-induced relaxation of the abdominal aorta in cannabinoid receptor 1 (CB-1) knock out mice. Therefore, we aimed to investigate the effect of ECS and the role of endothelial factors in the estrogen-induced vascular response.
Aim: To investigate the possible morphological and signaling changes underlying the enhanced estrogen-dependent relaxation observed in CB-1 receptor-deficient animals.
Methods: Our experiments were performed in CB-1 receptor knock out and wild type female mice. After anaesthesia (pentobarbital 50mg/kg ip.), abdominal aortic segments were isolated from the animals for previous myographic and histological as well as immunohistochemical (IHC) measurements in our study. The structure of the vessels investigated by hematoxylin-eosin (HE) and resorcin-fuchsin (RF) stained sections, and proteins involved in the signaling process were examined by immunohistochemical labeling (estrogen receptor (ER), thromboxane receptor (TP) and endothelial nitric oxide synthase (eNOS) and cyclooxygenase 2 (COX-2).
RESULTS: In HE staining, the intima-media ratio of CB-1 KO group was significantly lower compared to the control group (p<0.05). No significant difference in ER receptor and TP receptor density was found between the two groups. COX-2 density was significantly lower in the CB-1 KO group compared to the control group (p<0.05). eNOS density was significantly higher in the CB-1 KO group (p<0.001).
CONCLUSION: CB-1-KO mice are characterized by increased estrogen-induced vasorelaxation, which our results suggest may be associated with increased production of endothelial NO (nitric oxide); presumably associated with decreased levels of the constrictor prostanoids.
Funding: OTKA K116954 & K139231

University and Doctoral School

Semmelweis University, Doctoral School of Theoretical and Translational Medicine

Supervisor

Eszter Mária Horváth; Mária Szekeres

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6054

Start

10:30

End

10:35

Authors (legacy)

Bálint Bányai1; Stella Tímea Kiss1,2; Gabriella Dörnyei2; Mária Szekeres2; Eszter Mária Horváth1
1- Semmelweis University Department of Physiology Budapest
2- Semmelweis University Morfological and physiological institute Budapest