PhD Scientific Days 2024

Theoretical and Translational Medicine I.

Modulated Electro-hyperthermia Accelerates Tumour Delivery of Doxorubicin from Lyso-thermosensitive Liposome and Improves its Anticancer Activity in 4T1-Bearing Mice

Előadó neve

Aloss, Kenan, PhD

Neptun code

UL1GAP

Előadó munkahelye

Semmelweis University-Translational Medicine Institute

Előadó telefonszáma

06302411221

Előadó e-mail címe

kenan.aloss@phd.semmelweis.hu

Az előadás címe

Modulated Electro-hyperthermia Accelerates Tumour Delivery of Doxorubicin from Lyso-thermosensitive Liposome and Improves its Anticancer Activity in 4T1-Bearing Mice

Szerző(k) neve és munkahelye

Kenan Aloss¹, Syeda Mahak Zahra Bokhari¹, Pedro Henrique Leroy Viana¹, Nino Giunashvili¹, Csaba András Schvarcz¹, Gábor Szénási¹, Zoltán Benyó¹, Péter Hamar¹

1: Institute of Translational Medicine, Semmelweis University, 1094 Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Theoretical and Translational Medicine I.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction and Aim:
Modulated electro-hyperthermia (mEHT) is an advanced option in the hyperthermia field, applying 13.56 Radiofrequency electromagnetic current. By the autofocusing of mEHT the selective and significant (+2.5°C) heating of even deeply situated tumors is possible. In this study, we investigated the possibility of using mEHT as a source of heat for thermosensitive liposomes (TSLs) to effectively enhance the delivery and in vivo efficacy of doxorubicin (DOX).
Methods:
a triple-negative breast cancer cell line (4T1), was orthotopically injected into Balb/C mice and treated with a combination of mEHT and lyso- thermosensitive liposomal doxorubicin (LTLD). Tumor growth was followed by ultrasound and caliper. DOX accumulation in the tumor was assessed by in vivo imaging system (IVIS).
Results:
LTLD+ mEHT combination was more effective in tumor growth inhibition compared to free DOX and pegylated liposomal DOX (PLD) as demonstrated by both tumor volume and tumor weight reduction. Furthermore, LTLD combined with mEHT resulted in the highest DOX accumulation in the tumor at 1 h after treatment. Moreover, cancer cell damage was associated with upregulation of cleaved caspase-3 (cC3) in the damaged area, and reduction of Ki67 in the living area of (mEHT+ LTLD)- treated tumors.
Conclusion:
LTLD combined with mEHT represents a novel approach for DOX delivery into cancer tissue.
Funding:
ID: ÚNKP-23-3-II-SE-45, SE250+ EFOP-3.6.3-VEKOP-16-2017-00009, Tempus Stipendium Hungaricum

University

Semmelweis University

Supervisor

Peter Hamar

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6828

Start

09:45

End

09:55