PhD Scientific Days 2024

Poster Session L - Health Sciences 1.

Analysis of Chondroitin Sulfate and Heparan Sulfate Glycosaminoglycans in Human Tissues with Different Type of Lung Cancer

Előadó neve

Pál, Domonkos

Neptun code

A1LAY2

Előadó munkahelye

MTA-TTK Lendület (Momentum) Glycan Biomarker Research Group, Institute of Organic Chemistry, HUN-REN Research Centre for Natural Sciences

Előadó telefonszáma

+36 20 932 6072

Előadó e-mail címe

pal.domonkos@phd.semmelweis.hu

Az előadás címe

Analysis of Chondroitin Sulfate and Heparan Sulfate Glycosaminoglycans in Human Tissues with Different Type of Lung Cancer

Szerző(k) neve és munkahelye

Pál Domonkos, Lilla Turiák¹

1: MTA-TTK Lendület (Momentum) Glycan Biomarker Research Group, Institute of Organic Chemistry, HUN-REN Research Centre for Natural Sciences

Bemutatás módja

Poszter

Szekció

Poster Session L - Health Sciences 1.

Language of the presentation

English

Preferred session

Health Sciences

Összefoglaló szövege

Analysis of Chondroitin Sulfate and Heparan Sulfate Glycosaminoglycans in Human Tissues with Different Type of Lung Cancer

Introduction:
Lung cancer, the second most commonly diagnosed cancer has the highest mortality rate among all tumor types. Carbohydrates are utilized as diagnostic biomarkers in various cancers. During tumor development, glycosaminoglycans (GAGs) undergo changes in their abundance and structure.
Aims:
Our objective was to conduct a comprehensive quantitative and qualitative glycomic study on samples of small cell lung cancer and different subtypes of non-small cell lung cancer, analyzing both tumor and tumor adjacent regions.
Methods:
GAG chains were digested using bacterial lyases in multiple cycles on the tissue surface. The resulting chondroitin sulfate (CS) and heparan sulfate (HS) disaccharides were extracted from the tissue surface and purified. The analysis was performed on self-packed capillary columns with hydrophilic interaction liquid chromatography and weak anion exchange (HILIC-WAX) mixed mode resin with ammonium formate salt gradient using mass spectrometry detection in negative ionization mode.
Results:
Significant changes were observed in the content and sulfation patterns of GAGs. Although the alterations between the lung tumor phenotypes were not as remarkable, some differences could be identified. The abundance of CS was doubled in tumor samples, while the total content of HS did not show significant changes. Additionally, the average degree of sulfation significantly increased in all examined tumor phenotypes. Comparing adenocarcinoma samples to other lung tumor phenotypes, the CS 6-O-/4-O-sulfation ratio was increased. O-sulfated HS components were elevated in the tumor samples.
Conclusion:
Our results emphasize the importance of exploring the role of GAGs in lung cancer development, as several alterations were identified between tumor and tumor-adjacent tissue samples, as well as among different lung tumor phenotypes.
Funding:
Funding from the National Research, Development and Innovation Office (FK131603) is acknowledged. PD acknowledges the Semmelweis 250+ Excellence PhD Scholarship.

E-mail: pal.domonkos@phd.semmelweis.hu
University: Semmelweis University
Supervisor: Dr. Lilla Turiák

University

Semmelweis University

Supervisor

Lilla Turiák

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7405

Start

16:05

End

16:08