PhD Scientific Days 2024

Poster Session R - Pharmaceutical Sciences and Health Technologies 2.

Development and testing of polymer-encapsulated, amine- functionalized iron-based contrast materials in animal model

Előadó neve

Dr. Ms. Heydari, Fatemeh

Neptun code

IHGY3E

Előadó munkahelye

Semmelweis University Department of Biophysics and Radiation Biology

Előadó telefonszáma

0614591500

Előadó e-mail címe

fatemeh.heydari@stud.semmelweis.hu

Az előadás címe

Development and testing of polymer-encapsulated, amine- functionalized iron-based contrast materials in animal model

Szerző(k) neve és munkahelye

Fatemeh Heydari¹, Laszlo Forgach¹, Zoltan Varga², Noemi Kovacs¹, Ildiko Horvath¹, Agnes Maria Ilosvai³, Ferenc Kristaly⁴, Lajos Daroczi⁵, Zoltan Kaleta⁶, Bela Viskolcz³, Miklos Nagy³, Laszlo Vanyorek³, Domokos Mathe⁷, Krisztian Szigeti¹

1: Semmelweis University Department of Biophysics and Radiation Biology
2: Research Center for Natural Sciences, ELKH, Budapest,
3: Institute of Chemistry, University of Miskolc
4: Institute of Mineralogy and Geology, University of Miskolc
5: Department of Solid State Physics, University of Debrecen
6: Institute of Organic Chemistry, Semmelweis University
7: In Vivo Imaging ACF, Hungarian Center of Excellence for Molecular Medicine, Szeged

Bemutatás módja

Poszter

Szekció

Poster Session R - Pharmaceutical Sciences and Health Technologies 2.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

INTRODUCTION: Magnetic nanoparticles (NPs), particularly superparamagnetic variants such as magnetite, maghemite, and various ferrite NPs, emerge as promising alternatives to traditional Magnetic Resonance Imaging (MRI) contrast agents (CAs). Their heightened specificity and biocompatibility make them attractive candidates. The escalating demand for stable and precisely tuned magnetic NPs in biomedical applications highlights their significance. However, the preparation of these NPs remains a persistent challenge.
METHODS: Two different solvothermal methods (12 h reflux and a 4 min microwave, MW) were used to synthesize amine-functionalized ferrite, superparamagnetic NPs, doped with Zn2+ and Cu2+ ions. To overcome stability problems in the colloidal phase, the ferrite NPs were embedded in polyvinylpyrrolidone and could be easily redispersed in water. The morphological characterization of the NPs was executed by High Resolution Transmission Electron Microscopy, Atomic Force Microscopy and Dynamic Light Scattering (DLS). For detecting the supramolecular interactions and crystalline structure, Fourier Transform Infrared Spectroscopy and X-ray Powder Diffraction was utilized. The in vitro and in vivo MRI measurements were performed with a PET/MR system.
RESULTS: In case of the Zn-doped NPs, the conventional synthesis (ZnFe2O4-NH2 Refl.) resulted in a more stable system as compared to the microwave-assisted synthesis (ZnFe2O4-NH2 MW). These results were supported by DLS and AFM measurements, as well as in vitro MRI measurements, where inhomogeneities in the signal were detected. The CuFe2O4-NH2 MW samples however showed increased colloidal stability as well as homogenous MRI signal in vitro and in vivo. After injection, consistent with other SPION NPs, both samples exhibit a concentrated presence in the hepatic region of the animals, with comparable biodistribution and pharmacokinetics suspected.
CONCLUSION: Our investigation shows that the ferrite NPs are a feasible candidate for a new generational, multimodal MRI CA. Their chemical properties, owning an -NH2 group holds great options for surface modifications with chelators for isotopes or fluorescent pigments for multimodal molecular imaging purposes. It must be highlighted that the preparation method as well as the nature of the applied precursors play a crucial role in the synthesis of a stable system.

University

Semmelweis University

Supervisor

Krisztian Szigeti

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8017

Start

16:05

End

06:08