PhD Scientific Days 2024

Poster Session O - Surgical Medicine

Exercise promotes functional liver regeneration through the AMPK pathway following partial hepatectomy in rats

Előadó neve

Dr. Kovács, Szabina

Neptun code

O0K9P1

Előadó munkahelye

Department of Surgery, Transplantation and Gastroenterology, Semmelweis University

Előadó telefonszáma

06308540673

Előadó e-mail címe

kovacs.szabina@stud.semmelweis.hu

Az előadás címe

Exercise promotes functional liver regeneration through the AMPK pathway following partial hepatectomy in rats

Szerző(k) neve és munkahelye

Szabina Kovács¹, Klára Lévay¹

1: Department of Surgery, Transplantation and Gastroenterology, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session O - Surgical Medicine

Language of the presentation

Hungarian

Preferred session

Surgical Medicine

Összefoglaló szövege

Introduction: The only curative treatment for locally extensive liver tumors is surgical resection. Insufficient future liver remnant after major hepatectomy, however, deteriorates the surgical outcome due to post-hepatectomy liver failure. Therefore, by enhancing the regenerative capacity of the liver physical prehabilitation can improve the postoperative outcome after hepatectomy. Yet, the molecular mechanisms are not fully understood. The adenosine monophosphate-activated protein kinase (AMPK) is a key molecule in liver regeneration that improves the course of liver diseases.
Aims: We aimed to investigate the role of exercise-induced AMPK in liver regeneration after partial hepatectomy.
Method: Male Wistar rats divided into sedentary (S), physical prehabilitation (P) and physical prehabilitation followed by AMPK inhibition (PI) groups underwent partial hepatectomy. Morphological regeneration was assessed by liver weight, Ki67 index and MR volumetry. Functional regeneration was evaluated by standard laboratory parameters, indocyanin green secretion and 99mTc-mebrofenin hepatobiliary scintigraphy. To investigate the postoperative outcome endotoxemia model was established.
Results: Hepatocyte proliferation (p < 0.01) and liver function (p < 0.001) improved, while mortality (p < 0.001) reduced in the P group compared to the S group. AMPK inhibition abolished the beneficial effect of physical prehabilitation on liver function (p < 0.001) and postoperative outcome (p < 0.001). However, morphological regeneration (p < 0.05) was more pronounced in the PI group compared to the P group.
Conclusion: Exercise-induced AMPK activation has a central role in functional liver regeneration. Further investigation is needed to develop exercise mimetics for vulnerable patients who can not participate in physical prehabilitation programs.
Funding: Semmelweis University 250+ PhD Excellence Grant (38973/DIDIT/2024); New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund (ÚNKP-22-3-I-SE-2)

University

Semmelweis University

Supervisor

András Fülöp

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8030

Start

16:05

End

16:08