PhD Scientific Days 2024

Pharmaceutical Sciences and Health Technologies I.

Enabling toxicokinetic characterisation of 2,3-epoxypropyl isopropyl ether in the rat by substantial sensitivity improvement of the MS/MS detection

Előadó neve

Ms. Aliz, Széles

Neptun code

D1K9I2

Előadó munkahelye

Doctoral College Semmelweis University (Hungary)

Előadó telefonszáma

+36204942641

Előadó e-mail címe

szeles.aliz@phd.semmelweis.hu

Az előadás címe

Enabling toxicokinetic characterisation of 2,3-epoxypropyl isopropyl ether in the rat by substantial sensitivity improvement of the MS/MS detection

Szerző(k) neve és munkahelye

Aliz Széles¹, Katalin Monostory², Tibor Renkecz³

1: Doctoral College Semmelweis University (Hungary); HUN-REN Research Centre for Natural Sciences (Hungary); Toxi-Coop Toxicological Research Centre (Hungary)
2: HUN-REN Research Centre for Natural Sciences (Hungary)
3: Toxi-Coop Toxicological Research Centre (Hungary)

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences and Health Technologies I.

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

2,3-epoxypropyl isopropyl ether is a frequently used material during epoxy resin manufacturing process. There is still little known about the attainable systemic concentration of such chemicals in laboratory animals and in the human body. Present work can provide valuable information to assist exposure assessment/biomonitoring in occupational safety studies.
The LC-MS/MS method used gradient elution [mobile phase A (0.5% formic acid in ultrapure water) B (0.5% formic acid in acetonitrile)] on a YMC-Triart C18 column. As internal standard tert-butyl glycidyl ether was used since no stable isotope labelled analogue of the analyte was available. The Shimadzu LCMS-8060 triple quadrupole tandem mass spectrometer was operated in MRM mode with APCI ion source and the ethylnitrilium adduct form of the precursor ion was selected for fragmentation. The LLOQ for the analyte was 0.01 µg/mL over a 0.01–10 µg/mL range. The rat plasma matrix did not cause any signal suppression. The mean recovery was 106 % and 94.6 % at high (8 µg/mL) and low (0.03 µg/mL) level of concentration. The plasma samples proved to be stable at 75C ± 10C for at least 26 days and can be subjected to 3 freeze-thaw cycles and for at least 4 hour-storage on benchtop at room temperature.
In a toxicokinetic (TK) study, a single dose (1000 mg/kg; 1500 mg/kg and 2000 mg/kg) of 2,3-epoxypropyl isopropyl ether was administered orally to rats and TK plasma sparse sampling was performed at 9 time-points. The TK evaluation results with the method performance characterization will be presented in details.

University

Semmelweis University

Supervisor

Katalin Monostory

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7412

Start

16:45

End

16:55