Poster Session S - Health Sciences 2.
Cao Cancelas, Alejandro, PhD
University of Santiago de Compostela
637968360
alejandro.cao.cancelas@usc.es
Advancements in Water Contaminant Detection: Enhancing Food Safety for Animals and Humans
Alejandro Cao Cancelas1
1: University of Santiago de Compostela
Poszter
Poster Session S - Health Sciences 2.
English
Health Sciences
Introduction
In recent years, a significant escalation in the presence of toxic substances in water has been observed, primarily attributed to the combined effects of climate change and human activities. This heightened contamination poses a substantial threat to the health and well-being of both wildlife and human populations. Among these toxic substances are cyanotoxins, produced by cyanobacteria, and pesticides. Therefore, an improvement in the detection methods of these substances is necessary to enhance the hygienic quality of drinking water and, consequently, the health of both humans and animals.
Aims
The main objective of this work is to develop a ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method to identify these contaminants in drinking water with a sensitivity greater than the limit concentration set by European food safety regulations.
Material and methods
To develop this method for the identification of cyanotoxins and pesticides using UHPC-MS/MS, certified reference standards of the most common cyanotoxins have been employed, specifically microcystins (MCs) LR, RR, YR, LA, LY, LF, and des-MC-LR, as well as cylindrospermopsin and anatoxin-A. Regarding pesticides, certified reference standards of the most common pesticides in the European Union, including Imidacloprid, Atrazine, Metolachlor, and Metazachlor, have been utilized.
Results
A short UHPLC-MS/MS method has been developed, enabling the identification of 9 cyanotoxins and 4 pesticides in drinking water. Additionally, the source conditions (electrospray ionization, ESI) for each molecule have been optimized to ensure adequate sensitivity. Furthermore, the method allows for the separation of L-phenylalanine, a common amino acid in nature, from anatoxin-A, as they share the same mass transitions.
Conclusion
This developed method enables the identification and quantification of the most common water contaminants, thereby enhancing food safety for both animals and humans.
Funding
The research leading to these results has received funding mainly from grant PID2020-112626RB-C21 funded by MCIN/AEI/10.13039/501100011033, modalities «Research Challenges» and «Knowledge Generation».
University of Santiago de Compostela
Natalia Vilariño
I do not give consent to the publication of my abstract on the website of the congress.
Szabad
elfogadva
poszter
nem rendelkezett róla
7978
16:00
16:03